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Establishing Reference Values for Peripheral Blood Neutrophil CD64 index and Monocyte HLA-DR of Healthy Adult

zhang, l.; Li, Y.; Zhong, H.; Huang, J.; Yin, M.

2025-11-02 infectious diseases
10.1101/2025.10.29.25339107 medRxiv
Show abstract

Neutrophil CD64 (nCD64) index and Monocyte HLA-DR (mHLADR) played an important role in the diagnosis of infection, but their levels in healthy adults have not been thoroughly investigated. This study established reference values for nCD64 index and mHLADR% in the peripheral blood from 285 healthy adults. All the subjects were grouped into subgroups according to sex (male and female) and age (14-51 years, 51-100 years). The analyses indicated age to be an important factor associated with changes in nCD64 index, which gradually increased from 14 to 100 years and showed a positive correlation with age (P < 0.0001). In contrast, no significant differences in mHLA-DR% were observed across gender or age groups (p > 0.05). Furthermore, no correlation was found between nCD64 index or mHLA-DR% and all the inflammatory derived indicators of blood routine (P > 0.05). In conclusion, the reference intervals established for the nCD64 index and mHLA-DR% in healthy adults may provide supportive information for monitoring immune status in the context of infectious diseases..

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