Spleen Transcriptome Analysis of C57BL/6 Mouse in Response to Hot Water Extract from Spent Mushroom Substrate of Ganoderma lucidum and Cyclophosphamide
Wang, Z.; Wang, A.; Li, J.; Liao, Z.; Sun, L.; Lin, Z.; Liu, Y.
Show abstract
Previously, we have indicated that Hot Water Extract (HWE) from Spent Mushroom Substrate (SMS) of Ganoderma lucidum enhanced immune function of normal mice, and improved antioxidant activity and enhanced immune function of immunosuppression mice induced by cyclophosphamide (Cy). Here we performed the high throughput RNA sequencing strategy using Illumina HiSeq 2000 to characterize the spleen transcriptome from normal (CK1), HWE-treated (CK2), Cy-treated (CY) and both high dose HWE and Cy-treated mice (CH). From the RNA Sequencing, total mapped reads of map to Gene in CK1, CK2, CY and CH was 54 759 942, 54 678 926, 44 728 132 and 54 006 596, respectively. And gene expression was significantly different among CK1 and CK2, CY and CH. Compared with CK1, the gene expression of Ugt1a6b was down-regulated in CK2 after HWE treated. In addition, compared with CY, multiple tumor suppressor or tumorigenesis genes were down-regulated, such as Cdkn1a, Cdkn1b, Mapk10, Vash1, and Tnc and other genes in CK2 and CH. Taken together, our study highlighted the spleen transcriptome profiles of C57BL/6 mouse in response to HWE from SMS of G. lucidum and Cy, and indicated that HWE can improve the immune function of the mouse and accelerated the recovery of immunosuppression in Cy-treated mice.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Decorin inhibits glucose-induced lens epithelial cell apoptosis via suppressing p22phox-p38 MAPK signaling pathway 97%
- In silico analysis and in planta production of recombinant ccl21/IL1β protein and characterization of its in vitro anti-tumor and immunogenic activity 97%
- Metabolomics and Transcriptomics unravel the mechanism of browning resistance in Agaricus bisporus 96%
Similar papers in this journal
- The combination of lipopolysaccharide and D-galactosamine administration show positive genotoxic effect in mice liver 97%
- Effects of different environmental intervention durations on the intestinal mucosal barrier and the brain-gut axis in rats with colorectal cancer 96%
- Enhanced expression of Dystrophin, IGF-1, CD44 and MYH3 in plasma and skeletal muscles including Diaphragm of mdx mice after oral administration of Neu REFIX Beta 1,3-1,6 glucan 95%
Similar papers in this journal
Similar papers in this journal
- Integrated bioinformatics analysis reveals novel key biomarkers and potential candidate small molecule drugs in gestational diabetes mellitus 98%
- Identification and verification of 3 key genes associated with survival and prognosis of patients with colon adenocarcinoma via integrated bioinformatics analysis 97%
- Testosterone improves muscle function of the extensor digitorum longus in rats with sepsis 96%
Similar papers in this journal
- Structural variability, expression profile and pharmacogenetics properties of TMPRSS2 gene as a potential target for COVID-19 therapy 95%
- Identification of differentially expressed genes in the longissimus dorsi muscle of Luchuan and Duroc pigs by transcriptome sequencing 95%
- Molecular pathways associated with Kallikrein 6 overexpression in colorectal cancer 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.