Interaction of citrate-stabilized gold nanoparticles with umbilical cord RBC

IntroductionUmbilical cord blood contains stem cells which are useful in chemotherapy, implantation; however, cord RBC is considered as a waste product. It is necessary to use the umbilical cord blood for neonatal blood transfusion to avoid diseases. Nowadays, nanoparticles are used in biomedical and pharmaceutical industry. Nanoparticles interact with blood via different routes. In addition, these nanoparticles used in biomedicine, drug delivery, bioimaging, and to increase the shelf life of RBC. Nevertheless, research of nanoparticle interaction with umbilical cord red blood cells is still insufficient. Thus, in the present study, interaction between umbilical cord blood-RBC and citrate-stabilized gold nanoparticles is studied. MethodsUmbilical cord rbc isolated from whole blood immediately after the collection using centrifuge. PBS buffer is used as a physiological media and washing reagent. After, interaction of isolated RBC (2% suspension) with different concentration of citrate stabilized gold nanoparticle were investigated using UV-Visible spectroscopy, Raman spectroscopy, and Interfacial tension measurement technique. ResultsUV-Visible spectroscopy is used to investigate the changes in Soret band and oxygenation bands (Q1 and Q2). Variation in absorption intensity is observed in umbilical cord RBC incubated citrate stabilized gold nanoparticles. However, there is no significant shift in Soret band and Q1, Q2 band of cord-RBC with Au nanoparticles. In case of Raman spectroscopy, enhancement in disulphide band region (526 cm-1 and 579 cm-1) and change in the spin state marker is investigated. While, interfacial tension decreases for 10 {micro}l and increases for 100 {micro}l and 200{micro}l of 40 nM AuNP. ConclusionThe umbilical cord rbc remains unaffected by 10 {micro}l of citrate-stabilized gold nanoparticles (40 nM).