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Dietary amino acid manipulations in the common marmoset Callithrix jacchus impacts serum metabolites and FGF21 levels

Fujita, Y.; Ishibuchi, T.; Uematsu, A.; Hayashi, T.; Obata, F.

2025-04-19 physiology
10.1101/2025.04.14.648667 bioRxiv
Show abstract

Restricting dietary protein intake has metabolic and physiological benefits for animals. Rodent studies have identified the involvement of a hormone fibroblast growth factor 21 (FGF21), which is upregulated by sensing amino acid scarcity. However, to what extent this mechanism is conserved in primates remains elusive. Using common marmosets Callithrix jacchus as a non-human primate model, we demonstrate a protocol for protein restriction and dietary amino acid manipulation. Low protein diet induces a decrease in blood urea nitrogen, altered plasma amino acid profiles, and an increase in plasma FGF21. Supplementation of purified amino acids to the diet suppresses plasma features of protein restriction. Our data provide a dietary intervention technique in marmosets and an insight into the evolutionarily conserved mechanism of FGF21 induction during protein restriction.

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