The different functions of exercise, supplementary resveratrol, and the combination of exercise and supplementary resveratrol in young and old SAMP8 mice liver

The senescence-accelerated mouse prone 8 (SAMP8) has about half the normal lifespan of a rodent. Therefore, we determined whether the different age degrees has rapid physiological senescence with the same interaction function and mechanism of exercise and supplementary resveratrol intake by SAMP8 mice liver. Histological of aging related liver disease was examined SAMP8 mice by hematoxylin-eosin and Massons trichrome staining. Apoptosis was determined by TUNEL staining. The mechanism proteins expression of p-PI3K/PI3K, p-Akt/Akt, Bad, cytochrome c, Bcl 2, ERK1, IL-6, STAT3, MEK5, p-ERK5/ERK5, FGF2, and MMP2/9 were examined by western blot. Results showed that the 3- and 6-month-old SAMP8 mice liver, the senescence-accelerated liver cross-sections observed adipocytes, collagen, and apoptosis cells appear, but lower occurs by exercise, supplementary resveratrol, and their combination. Combination exercise and supplementary resveratrol at the 6-month-old SAMP8 mice has significant increase ratio by p-PI3K/PI3K compared to 3-month-old SAMP8 mice liver (p<0.01), and compared with without treatment SAMP8 mice liver. Exercise, supplementary resveratrol intake, and combined of exercise and resveratrol has facilitated p-Akt/Akt ratio increases, and ERK1/Bcl2 increases, but Bad/Cytochrome c decreases at the 6-month-old SAMP8 by western blot. IL-6/STAT3/MEK5 and p-ERK5/ERK5 ratio have good functions at 6-month-old SAMP8 mice better than 3-month-old SAMP8. FGF2/MMP2 decreases and MMP9 increases observed at the 3-month-old SAMP8 by exercise, supplementary resveratrol, and combination. Indeed, we suggest supplementary resveratrol can help exercise therapy ageing related liver diseases.