Pre-analytical delay as a dominant confounder in blood RNA-seq: Rapid ex vivo gene expression changes in EDTA blood
Günther, K.;Andreou, I.;Kim, D.;Shaffer, J.;Sprenger-Haussels, M.
Show abstract
Although the impact of delayed processing on gene expression in EDTA blood has been well documented using targeted assays and microarray platforms, the emergence of next-generation RNA sequencing (RNA-seq) has not yet been leveraged to systematically compare these effects against stabilized whole blood collection systems. Notably, no study has performed a time course RNA-seq analysis with human bulk RNA of matched EDTA and PAXgene blood RNA samples drawn from the same subjects. EDTA is still widely used for gene expression analysis studies. Yet, the genome-wide dynamics by which EDTA blood transcriptomes deviate from a stabilized reference over time remain poorly defined. This represents an important methodological gap, given the increasing reliance on RNA-seq for biomarker discovery, clinical transcriptomics and diagnostics.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- EDTA tubes are suitable for insulin and C-peptide measurement in resource-limited settings and can be stored at room temperature for up to 24 hours 94%
- Exploring the Diagnostic Potential of miRNA Signatures in the Fabry Disease Serum: A Comparative Study of Automated and Manual Sample Isolations 94%
- Optimization Of Dried Blood Spot Assays For Hepatitis B Virus Surface Antibody Quantification 93%
Similar papers in this journal
- Delayed processing of blood samples impairs the accuracy of mRNA-based biomarkers 97%
- Hilab system, a new point-of-care hematology analyzer supported by the Internet of Things and Artificial Intelligence 94%
- Choice of lipid supplementation for in vitro erythroid cell culture impacts reticulocyte yield and characteristics 94%
Similar papers in this journal
- SARS-CoV-2 Spike protein binding of glycated serum albumin - its potential role in the pathogenesis of the COVID-19 clinical syndromes and bias towards individuals with pre-diabetes/type 2 diabetes & metabolic diseases 91%
- Metabolite patterns in human myeloid hematopoiesis result from lineage-dependent active metabolic pathways 91%
- Potential and Limitations of the MinION Nanopore array for miRNA-Enabled Early Cancer Detection 91%
Similar papers in this journal
- Detailed analysis of public RNAseq data and longnon-coding RNA: a proposed enhancement to mesenchymalstem cell characterisation 90%
- Performance Comparison Of Agilent New SureSelect All Exon v8 Probes With v7 Probes For Exome Sequencing 89%
- Using Published Pathway Figures in Enrichment Analysis and Machine Learning 89%
Similar papers in this journal
- Development and optimization of a diluted whole blood ELISpot assay to test immune function 92%
- The Quantification of Antibody Elements and Receptors subunit expression using qPCR: The Design of VH, VL, CH, CL, FcR subunits primers for a more holistic view of the immune system. 90%
- Normal human lymph node T follicular helper cells and germinal center B cells accessed via fine needle aspirations 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.