Pulse-wave harmonic signatures of cardiovascular regulatory coherence to a cognitive challenge in mild cognitive impairment: a community-based cross-sectional study

To evaluate whether hemodynamic responses to acute cognitive stress, measured via pulse-wave harmonic analysis, can characterize cardiovascular regulatory coherence and differentiate older adults with mild cognitive impairment (MCI) from cognitively intact individuals, this exploratory cross-sectional observational study utilized a within-session pre-post cognitive task design. A total of 101 community-dwelling older adults in southern Taiwan were stratified by Montreal Cognitive Assessment (MoCA) scores into Reference (MoCA [&ge;]26, n=12, paired n=10), MCI (MoCA 18-25; n=50, paired n=45), and dementia-level (MoCA <18; n=39) groups, the latter being excluded from task-evoked analyses. The primary outcome was the Harmonic Response Consistency Score (HRCS), quantifying the directional uniformity of cardiovascular regulatory responses, alongside secondary measures of harmonic amplitudes (Cn) and phase angles (Pn). Although mean pre-post changes were subtle, response organization differed by cognitive status. The Reference group exhibited high response consistency (mean HRCS = 9.00), characterized by coordinated harmonic down-modulation. Conversely, the MCI group showed attenuated, directionally heterogeneous responses. Compared to the Reference group, the MCI group demonstrated significantly lower HRCS values for the Cn domain (Mean difference: 2.60, 95% CI 0.29-4.91; p=0.020) and PnSD domain (Mean difference: 1.98, 95% CI 0.04-3.92; p=0.030), indicating a breakdown in regulatory coherence. These findings suggest that acute cognitive stimulus reveals coherent harmonic down-modulation in cognitively intact older adults but fragmented responses in MCI. Pulse-harmonic profiling thus serves as a robust physiological index of cardiovascular regulatory coherence, which, when integrated with neuropsychological assessments, may enhance the sensitivity of non-invasive, community-based screening frameworks for early cognitive aging.