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Immunogenicity of different types of adjuvants in bacterial vaccine

Li, Y.-Y.; Wang, N.; Han, Q.; Feng, T.; Zhu, Z.; Xu, T.-L.; Quan, K.-J.; Zhang, G. F.

2025-12-29 microbiology
10.64898/2025.12.28.696776 bioRxiv
Show abstract

This study aims to investigate four common trivalent avian infectious coryza (AIC) vaccines available on the market. It detects the antibody levels of three serotypes at 7 days and 14 days after the first immunization, and at 30 days, 90 days, and 180 days after the second immunization using HI (Hemagglutination Inhibition Test) and ELISA (Enzyme-Linked Immunosorbent Assay). Additionally, the levels of cytokines including IFN-{gamma} (Interferon-Gamma), IL-2 (Interleukin-2), and IL-3 (Interleukin-3) are measured. Furthermore, the protective rates of four of these vaccines are verified 180 days later. It demonstrated that the immunogenicity of mineral oil adjuvanted vaccine is higher than that of Aluminum adjuvanted. The animals should be boosted with another booster immunization six month post the first immunization to protect them from infectious coryza.

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