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Dynamic programming model for pattern recognition on the PfHRP2 sequence variants: a smart approach to improve malaria immunodiagnostics

Okeke, I.; Ajima, U.

2025-08-19 infectious diseases
10.1101/2025.08.15.25333763 medRxiv
Show abstract

The issue of poor diagnostic outcomes with malaria rapid diagnostic tests (mRDTs) is a serious concern. The World Health Organization (WHO) recommends confirming all suspected malaria cases through parasite-based diagnostic testing to ensure early detection, timely treatment, and minimize transmission risks. The mRDTs are crucial tools for this purpose and should consistently provide reliable results to ensure that patients receive appropriate treatment. In this study, a Python-based dynamic programming algorithm for recognizing unique patterns on genomic sequences was scripted and implemented to discover the presence, range, and frequency of epitope motifs in the genomic sequences of different PfHRP2 isolates. Twenty three amino acid sequence variants of PfHRP2 obtained from various databases were investigated. The investigations revealed that the majority of the unreviewed sequences had an abundance of different PfHRP2 epitope motifs at multiple sites, whereas these motifs were scarce among the reviewed sequences.

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