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A Study on Antibiotic Susceptibility Pattern of DNase Positive Staphylococci Isolated from Selected Libraries of Halls of Residence in Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.

Olaiya, V. O.

2025-06-30 public and global health
10.1101/2025.06.29.25330498 medRxiv
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IntroductionDNase-positive staphylococci are clinically significant pathogens capable of causing community-acquired infections. This study assessed their prevalence and antibiotic resistance patterns on reading tables in student halls of residence at Obafemi Awolowo University, Ile-Ife, Nigeria. Materials and MethodsSixty-eight tables across four halls were swabbed. Isolates were identified as presumptive staphylococci via Gram staining (cocci in clusters), catalase positivity, and oxidase negativity. DNase testing confirmed 12 isolates from 23 staphylococci. Kirby-Bauer disc diffusion tested susceptibility to seven antibiotics (rifampicin, cefoxitin, erythromycin, clindamycin, chloramphenicol, tetracycline, ciprofloxacin) using CLSI (2016) standards. ResultsAmong DNase-positive isolates, resistance rates were: tetracycline (42%, 5/12), chloramphenicol (33%, 4/12), erythromycin (17%, 2/12), and rifampicin/cefoxitin/clindamycin (8% each, 1/12). All isolates (100%) were susceptible to ciprofloxacin. Discussion/ConclusionReading tables serve as reservoirs for antibiotic-resistant staphylococci, linked to inadequate cleaning. Ciprofloxacin showed maximal efficacy, but judicious use is advised. Regular surface disinfection is critical to prevent community transmission in academic environments.

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