SRS microscopy identifies inhibition of vitellogenesis as a mediator of lifespan extension by caloric restriction in C. elegans
Yang, B.; Manifold, B.; Han, W.; DeSousa, C.; Zhu, W.; Streets, A. M.; Titov, D. V.
10.1101/2025.01.31.636008 bioRxivShow abstract
The molecular mechanisms of aging are not fully understood. Here, we used label-free Stimulated Raman scattering (SRS) microscopy to investigate changes in proteins and lipids throughout the lifespan of C. elegans. We observed a dramatic buildup of proteins within the body cavity or pseudocoelom of aged adults that was blunted by interventions that extend lifespan: caloric restriction (CR) and the reduced insulin/insulin-like growth factor signaling (IIS) pathway. Using a combination of microscopy, proteomic analysis, and validation with mutant strains, we identified vitellogenins as the key molecular components of the protein buildup in the pseudocoelom. Vitellogenins shuttle nutrients from intestine to embryos and are homologous to human apolipoprotein B, the causal driver of cardiovascular disease. We then showed that CR and knockdown of vitellogenins both extend lifespan by >60%, but their combination has no additional effect on lifespan, suggesting that CR extends the lifespan of C. elegans in part by inhibiting vitellogenesis. The extensive dataset of more than 12,000 images stitched into over 350 whole-animal SRS images of C. elegans at different ages and subjected to different longevity intervention will be a valuable resource for researchers interested in aging.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- fmo-4 promotes longevity and stress resistance via ER to mitochondria calcium regulation in C. elegans 95%
- Metabolic stress is a primary pathogenic event in transgenic Caenorhabditis elegans expressing neuronal human amyloid-β 95%
- Automatically tracking feeding behavior in populations of foraging C. elegans 95%
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
- LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays 94%
- DAF-2/insulin IGF-1 receptor regulates mobility during ageing by integrating opposite inputs from muscle and neuronal tissues 94%
- Reduction of DNA Topoisomerase Top2 reprograms the epigenetic landscape and extends health and life span across species 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.