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A method for inducing a senescence trend in mesenchymal stem cells using D-galactose

Ji, X.; Zhou, X.; Chen, T.; Duan, C.; Tian, W.; Zhu, L.; Ma, Y.; Gao, L.

2025-01-22 cell biology
10.1101/2025.01.19.633537 bioRxiv
Show abstract

The aging of the body is accompanied by a decline in tissue function, and this decline mainly stems from the aging of tissue stem cells. Therefore, they play a crucial role in the aging of the body.This study explores the biological characteristics of senescent hUCMSCs by establishing a replicative senescence model of hUCMSCs and a rapid senescence model induced by D - galactose (D-Gal), and analyzing the proliferative capacity, cell viability, cell cycle, pluripotency, and reactive oxygen species (ROS) levels of senescent hUCMSCs.

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