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The clots removed from ischaemic stroke patients by mechanical thrombectomy are amyloid in nature

Grixti, J. M.; Chandran, A.; Pretorius, J.-H.; Walker, M.; Sekhar, A.; Pretorius, E.; Kell, D. B.

2024-11-04 cardiovascular medicine
10.1101/2024.11.01.24316555 medRxiv
Show abstract

Ischemic stroke due to large vessel occlusion results from the blockage of a major cerebral artery by a clot; however, the origins and molecular composition of these clots remain poorly understood. Mechanical thrombectomy has become a standard treatment to remove obstructive clots, providing a unique opportunity to analyze their properties. We previously demonstrated that blood can clot into an amyloid-like form, generating fibrinaloid microclots (2-200 m) that are highly resistant to fibrinolysis. In this study, archived clots from eight ischemic stroke patients with large vessel occlusion were examined, using samples stored in the Walton Centre Clot Bank in Liverpool, UK. All clots exhibited strong, heterogeneous amyloid staining, revealing a pervasive amyloid component. These findings represent a previously unreported characteristic of stroke clots, highlighting the potential for amyloid-targeted therapies to overcome their fibrinolytic resistance and providing a foundational new insight into ischaemic stroke pathophysiology and treatment.

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