Novel Imaging Tools to Study Mitochondrial Dynamics in Caenorhabditis elegans
Valera-Alberni, M.; Yao, P.; Romero-Sanz, S.; Lanjuin, A.; Mair, W. B.
Show abstract
Mitochondria exhibit a close interplay between their structure and function. Understanding this intricate relationship requires advanced imaging techniques that can capture the dynamic nature of mitochondria and their impact on cellular processes. However, much of the work on mitochondrial dynamics has been done in single celled organisms or in vitro cell culture. Here, we introduce novel genetic tools for live imaging of mitochondrial networks in the nematode Caenorhabditis elegans, addressing a pressing need for advanced techniques in studying organelle dynamics within live intact multicellular organisms. Through a comprehensive analysis, we directly compare our tools with existing methods, demonstrating their advantages for visualizing mitochondrial morphology and contrasting their impact on organismal physiology. We reveal limitations of conventional techniques, while showcasing the utility and versatility of our approaches, including endogenous CRISPR tags and ectopic labeling. By providing a guide for selecting the most suitable tools based on experimental goals, our work advances mitochondrial research in C. elegans and enhances the strategic integration of diverse imaging modalities for a holistic understanding of organelle dynamics in living organisms.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- From pathogen to commensal to probiotic: modification of Microbacterium nematophilum-C. elegans interaction during chronic infection by the absence of host insulin signalling 94%
- The impact of rearing environment on C. elegans: Phenotypic, transcriptomic and intergenerational responses to 3D enriched habitats 94%
- An ALS-associated mutation in human FUS reduces neurotransmission from C. elegans motor neurons to muscles 93%
Similar papers in this journal
- Conditional immobilization for live imaging C. elegans using auxin-dependent protein depletion 96%
- Distinct mechanoreceptor pezo-1 isoforms modulate food intake in the nematode Caenorhabditis elegans 95%
- Escherichia coli Metabolite Profiling Leads to the Development of an RNA Interference Strain for Caenorhabditis elegans 95%
Similar papers in this journal
- Precise Lineage Tracking Using Molecular Barcodes Demonstrates Fitness Trade-offs for Ivermectin Resistance in Nematodes 93%
- Oocyte surface proteins EGG-1 and EGG-2 are required for eggshell integrity in Caenorhabditis elegans 93%
- The RU486-dependent activation of the GeneSwitch system in adult muscles leads to severe adverse effects in Drosophila 93%
Similar papers in this journal
- Measuring nucleus mechanics within a living multicellular organism: Physical decoupling and attenuated recovery rate are physiological protective mechanisms of the cell nucleus under high mechanical load 92%
- CentTracker: a trainable, machine learning-based tool for large-scale analyses of C. elegans germline stem cell mitosis 92%
- Basal autophagy during meiotic prophase I is required for accurate chromosome segregation in Drosophila oocytes and declines during oocyte aging 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.