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Identification of a P62-TIF-IA axis that drives nucleolar fusion and the senescence associated secretory phenotype

Thoms, H. C.; Brant, T.; Duckett, K.; Yang, Y.; Dong, J.; Wang, H.; Derby, F.; Akeke, T.; Mann, D.; Millar, F. R.; Von Kriegsheim, A.; Acosta, J. C.; Oakley, F.; Stark, L. A.

2023-12-06 molecular biology
10.1101/2023.12.05.570133 bioRxiv
Show abstract

Two key characteristics of senescent cells are nucleolar fusion and secretion of a plethora of pro-inflammatory cytokines called the senescence-associated secretory phenotype (SASP). The SASP is dependent on NF-{kappa}B but the initial trigger, and links with nucleoli, are unclear. Using multiple in vitro and in vivo models, we show that an early response to oncogene- and therapy-induced senescence (OIS and TIS) is nuclear/nucleolar accumulation of the PolI complex component, TIF-IA. This accumulation is essential for nucleolar fusion, the SASP and senescence, independent of rDNA transcription. We show that in steady state, TIF-IA is targeted for autophagic degradation by the p62 cargo receptor and that accumulation in senescence occurs as a consequence of ATM activation, which disrupts the p62-TIF-IA interaction. In mice, TIF-IA accumulates in colonic mucosa with age, which is further enhanced in the nfkb1-/- model of accelerated ageing. Together, these results reveal a p62-TIF-IA nucleolar stress axis that regulates the SASP and senescence, and that warrants further investigation as an anti-ageing target.

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