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scRNA-seq revealed the special IG H&L V(D)J allelic inclusion rearrangement and the high proportion dual BCR expressing B cells
2023-04-18
allergy and immunology
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Since the first report of V (D) J " allelic exclusion/inclusion"and" dual BCR "in 1961, despite continuous new discoveries, the proportion and source mechanism of dual BCR under physiological conditions have been puzzling immuologists.This study takes advantage of the single cell V (D) J sequencing technology, which can perfectly match the heavy and light chains of BCR at the level of a single B cell, and obtain the full length mRNA sequence of the CDR3 region. By analyzing the pairing of functi...
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