Analysis of somatic mutations in senescent cells using single-cell whole-genome sequencing
Zhang, L.; De Cecco, M.; Lee, M.; Hao, X.; Maslov, A. Y.; Montagna, C.; Campisi, J.; Dong, X.; Sedivy, J. M.; Vijg, J.
Show abstract
SO_SCPLOWUMMARYC_SCPLOWSomatic mutations accumulate in multiple organs and tissues during aging and are a known cause of cancer. Here we tested whether mutations accumulate during replicative senescence. Cellular senescence is also a possible cause of functional decline in aging, yet also acts as an anti-cancer mechanism in vivo. Using single-cell whole-genome sequencing, we compared mutation burdens between early passage and deeply senescent human fibroblasts. The results showed that single-nucleotide variations and small insertions and deletions increased in senescent cells by about two-fold, but have the same spectrum as early passage cells, while it has been known that particular mutational signatures are found in tumor cells. In contrast, aneuploidies were observed in half the senescent cells, but largely absent in early passage cells. Thus, the patterns of mutations among senescent, normal-aged and tumor cells differ significantly.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- A concerted increase in readthrough and intron retention drives transposon expression during aging and senescence 96%
- Revisiting the Hayflick Limit: Insights from an Integrated Analysis of Changing Transcripts, Proteins, Metabolites and Chromatin 94%
- Short senolytic or senostatic interventions rescue progression of radiation-induced frailty and premature ageing in mice 94%
Similar papers in this journal
- Cell-Surface LAMP1 is a Senescence Marker in Aging and Idiopathic Pulmonary Fibrosis 94%
- Senescent cells enhance newt limb regeneration by promoting muscle dedifferentiation 94%
- A genome-wide screen identifies genes that suppress the accumulation of spontaneous mutations in young and aged yeast cells 93%
Similar papers in this journal
- Under the shadow: Old-biased genes are subject to weak purifying selection at both the tissue and cell type-specific levels 93%
- Concurrent evolution of anti-aging gene duplications and cellular phenotypes in long-lived turtles 91%
- Comparative study of protein aggregation propensity and mutation tolerance between naked mole-rat and mouse 91%
Similar papers in this journal
- A lysosomal dimmer switch regulates cellular quiescence depth 93%
- Systematic Approach Identifies Multiple Transcription Factor Perturbations That Rejuvenate Replicatively Aged Human Skin Fibroblasts 92%
- Dpp and Immune Response Pathways Factors Mediate Paracrine Induction of Senescent Cells in Drosophila 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.