Rapid and Live-cell Detection of Senescence in Mesenchymal Stem Cells by Micro Magnetic Resonance Relaxometry
Thamarath, S. S.; Tee, C. A.; Neo, S. H.; Yang, D.; Othman, R. B.; Boyer, L.; Han, J.
Show abstract
Detection of cellular senescence is important quality analytics for cell therapy products, including mesenchymal stromal cells (MSCs). However, their detection is critically limited by the lack of specific markers and the destructive assays used to read out these markers. Here, we establish a rapid, live-cell assay for detecting senescent cells using heterogeneous mesenchymal stromal cell (MSC) cultures. We report that the T2 relaxation time measured by microscale Magnetic Resonance Relaxometry ({micro}MRR), which is related to intracellular iron accumulation, correlates strongly with senescent markers in MSC cultures under diverse conditions including different passages and donors, size-sorted MSCs by inertial spiral microfluidic device, and drug-induced senescence. In addition, the live-cell and non-destructive method presented here has general applicability to other cells and tissues, and can critically advance our understanding of cellular senescence.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Therapeutic effects of PDGF-AB/BB against cellular senescence in human intervertebral disc 94%
- Metformin alleviates aging-associated cellular senescence of human adipose stem cells and derived adipocytes 91%
- Increase of cell surface vimentin (CSV) is associated with vimentin network disruption and subsequent stress-induced premature senescence (SIPS) in human chondrocytes 91%
Similar papers in this journal
- Single-cell fluorescence imaging reveals heterogeneity in senescence biomarkers and identifies rapamycin-responsive sub-populations 94%
- Senolytic-Resistant Senescent Cells Have a Distinct SASP Profile and Functional Impact: The Path to Developing Senosensitizers 92%
- Blood-brain barrier dysfunction promotes astrocyte senescence through albumin-induced TGFβ signaling activation 92%
Similar papers in this journal
- Comprehensive Bulk and Single-Cell RNA Sequencing Uncovers Senescence-Associated Biomarkers in Therapeutic Mesenchymal Stem Cells 95%
- Phenotypic and functional characterization of corneal endothelial cells during in vitro expansion 92%
- Generation of Somatic Mitochondrial DNA-Replaced Cells for Mitochondrial Dysfunction Treatment 92%
Similar papers in this journal
- Exploring Breast Cancer-Related Biochemical Changes in Circulating Extracellular Vesicles Using Raman Spectroscopy 90%
- A Mem-dELISA platform for dual color and ultrasensitive digital detection of colocalized proteins on extracellular vesicles 88%
- Multi-Omics Analysis of Magnetically Levitated Plasma Biomolecules 88%
Similar papers in this journal
- A Fully-Automated Senescence Test (FAST) for the high-throughput quantification of senescence-associated markers 94%
- Substrate Stiffness Dictates Unique Doxorubicin-induced Senescence-associated Secretory Phenotypes and Transcriptomic Signatures in Human Pulmonary Fibroblasts 93%
- Multiplexed single-cell imaging reveals diverging subpopulations with distinct senescence phenotypes during long-term senescence induction. 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.