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Reduced ech-6 Expression Attenuates Fat-induced Premature Aging in C. elegans

Liu, Y. J.; Gao, A. W.; Smith, R. L.; Janssens, G. E.; Panneman, D. M.; Jongejan, A.; van Weeghel, M.; Vaz, F. M.; Silvestrini, M. J.; Lapierre, L. R.; MacInnes, A. W.; Houtkooper, R. H.

2020-11-02 cell biology
10.1101/2020.11.02.364760 bioRxiv
Show abstract

Deregulated energy homeostasis represents a hallmark of aging and results from complex gene-by-environment interactions. Here, we discovered that reducing the expression of the gene ech-6 encoding enoyl-CoA hydratase remitted fat diet-induced deleterious effects on lifespan in Caenorhabditis elegans, while a basal expression of ech-6 was important for survival under normal dietary conditions. Lipidomics revealed that supplementation of fat in ech-6-silenced worms had marginal effects on lipid profiles, suggesting an alternative fat utilization for energy production. Transcriptomics further suggest a causal relation between the lysosomal pathway, energy production, and the longevity effect conferred by the interaction between ech-6 and high-fat diets. Indeed, enhancing energy production from endogenous fat by overexpressing lysosomal lipase lipl-4 recapitulated the lifespan effects of high-fat diets on ech-6-silenced worms. Collectively, these results reveal that the gene ech-6 modulates metabolic flexibility and may be a target for promoting metabolic health and longevity.

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