In-vivo evaluation of the anti-diarrheal effect of Lactococcus lactis subspecies lactis and Lactococcus piscium isolated from yogurt.

Lactobacillus and Lactococcus species found in the yogurt of different sources are most widely assayed and used all over the world as a probiotic agent. This study aimed to isolate and identify novel probiotic agents with therapeutic value against diarrhea. Initially, the probiotic properties of the isolated lactic acid bacteria from the yogurt samples of the Chittagong division, Bangladesh, were evaluated. All probiotic candidates inhibited the growth of selected pathogens, including Escheriachia coli, Serratia sp. Salmonella paratyphi, Streptococcus Group-B, Staphylococcus aureus, Haemophillus influenzae, Bacillus subtillis, and Klebsiella pneumoniae. Lactococcus lactis subsp lactis were found most useful in inhibiting all the selected pathogens. When the probiotics were applied against castor oil-induced diarrhea in the rat model, Lactococcus lactis subspecies lactis and Lactococcus piscium were found significantly effective relative to the controls indicating their potentiality as an alternative therapeutic against diarrhea. HighlightsO_LILactococcus lactis subspecies lactis and Lactococcus piscium has shown potentiality to be a therapeutic agent against castor-oil induced diarrhea in an animal model. C_LIO_LILactococcus lactis subspecies lactis and Lactococcus piscium inhibited the growth of specified pathogens. C_LI