Primates

Behavioral thermoregulation allows animals to reduce the physiological costs associated with variation in the thermal environment, yet little is known about how chimpanzees respond behaviorally to the cool and humid conditions of tropical montane forests. We examined how microclimatic conditions influenced resting posture, sun/shade use, substrate use, vertical position within the canopy, and day nest use in eastern chimpanzees (Pan troglodytes schweinfurthii) in Nyungwe National Park, Rwanda. From March 2024 to February 2025, we collected 5,661 individual behavioral records during 486 contact hours and paired behavioral observations with measurements of ambient temperature, water vapor pressure, wind speed, and rainfall. Bayesian mixed models showed that temperature was the most consistent climatic correlate of resting behavior. Under cooler conditions, chimpanzees were more likely to adopt heat conserving postures, particularly curled and huddled postures, and to use day nests. As temperature increased, chimpanzees increased their use of shade, shifted from arboreal to terrestrial resting, and used lower canopy strata more frequently. Higher wind speed was also associated with reduced use of the upper canopy, whereas rainfall, water vapor pressure, and fruit availability generally showed weaker or behavior-specific associations. These findings indicate that chimpanzees in a cool montane forest flexibly adjust posture, solar exposure, substrate use, vertical position, and day nest use in relation to local thermal conditions. Resting behaviour may therefore provide an important context for behavioral thermoregulation, extending our understanding of chimpanzee thermal ecology beyond the warmer lowland and savanna habitats that have received most previous attention.

The human larynx, compared to those of closely related primates, lies deeper in the throat and lacks vocal membranes and air sacs. These shifts are usually analyzed regarding their acoustic effects on vowel-like vocalizations, since the evolution of speech was long thought to require an expansion of vocal range driven by vocal tract modifications. However, vowels are just one type of phoneme, and speech is just one class of human utterance. To understand the evolutionary underpinnings of known shifts in human vocal morphology, a broader bioacoustic comparison is needed. Specifically, the range of sounds used in human speech must be compared to that employed in other human vocalizations and in the repertoires of extant close primate relatives. Here, we measure the acoustic-feature space occupied by human speech, non-linguistic, and musical vocalizations along with the calls of chimpanzees, bonobos, and chacma baboons. We use Mel-frequency cepstral coefficients to create an acoustic space depicting the spectro-temporal features of over 750,000 brief vocal segments sourced from published databases and other verified sources. Speech and song occupied significantly less volume in this acoustic space than human non-linguistic vocalizations. In addition, the acoustic-feature volumes of speech and song were not statistically distinct from those of non-human primates. These results suggest that speech was not enabled by an expansion of human vocal acoustic space. Anatomical shifts unique to humans may have led to an elaboration of non-linguistic utterances, but learned vocalizations use a surprisingly small fraction of this space. Our understanding of human vocal evolution will be further informed by additional systematic comparisons of the function and homology of non-speech vocalizations, along with the collection and incorporation of more complete non-human primate vocal datasets, especially from Gorilla and Orangutan.

A remnant Atlantic Forest fragment within Sao Paulos urban environment supports six mammal species, with Didelphis being most abundant, while maintaining natural temporal activity patterns and successful reproduction, demonstrating the conservation value of small protected areas within megacities for preserving native wildlife despite intense anthropic pressure. The Forest Reserve of the Institute of Biosciences (FRIB) is a remnant of the Atlantic Forest located at the University of Sao Paulo, city of Sao Paulo campus, in Brazil. Despite urban pressures such as altered food resources, pollution, pathogens, and interactions with invasive and domestic species, several wildlife species persist, demonstrating adaptability to urban environments. This study aimed to sample the mammal species diversity of FRIB using camera traps. A total of 367 records of six mammal species were obtained over 107 days of sampling, between June and November 2024, resulting in 7,872 hours of sampling effort. The species recorded were Callithrix jacchus, Callithrix penicillata, Didelphis aurita, Didelphis albiventris, and unidentified species of the orders Chiroptera and Rodentia. Didelphis sp. was the most recorded genera with 201 records. These results highlight the significance of this forest fragment in protecting wild animals in an environment facing intense anthropic pressure. ResumoA Reserva Florestal do Instituto de Biociencias (FRIB) e um remanescente da Mata Atlantica situado na Universidade de Sao Paulo, em Sao Paulo, Brasil. Apesar das pressoes urbanas, como mudancas nos recursos alimentares, poluicao, patogenos e interacoes com especies invasoras ou domesticas, diversas especies de animais selvagens continuam existindo, evidenciando sua capacidade de adaptacao aos ambientes urbanos. O objetivo deste estudo foi amostrar a diversidade de especies de mamiferos da FRIB atraves de armadilhas fotograficas. Durante um periodo de 107 dias de amostragem, de junho e novembro de 2024, foram obtidos 367 registros de seis especies diferentes de mamiferos, totalizando 7.872 horas de esforco. As especies registradas foram Callithrix jacchus, Callithrix penicillata, Didelphis aurita, Didelphis albiventris, alem de especies nao identificadas das ordens Chiroptera e Rodentia. Didelphis sp. foi o genero com maior numero de registros (n=201). Esses resultados ressaltam a importancia desse fragmento florestal na protecao da fauna selvagem em um ambiente que enfrenta intensa pressao antropica.

Among Madagascar primates, the sportive lemurs (family Lepilemuridae) have seen their species diversity increase from eight in 2005 to 26 in 2009 mostly by applying the phylogenetic species concept to DNA barcode data. Despite the genus being speciose, only one case of sympatry is known from northern Madagascar, where two sportive lemur species described based on low mtDNA divergence, Lepilemur ankaranensis and Lepilemur milanoii, were found to co-occur at the center of their joint distribution range. Here, to clarify the taxonomy of these two species and examine their sympatry, we apply an integrative taxonomic framework to genomic and morphological data from 84 individuals of L. ankaranensis and L. milanoii, encompassing their entire distribution range and the forest of Analafiana, beyond their southernmost limit. Using clustering, multivariate, and isolation by distance analyses, we find no evidence of a sympatric zone and show that despite clear genetic differentiation between regions, the genomic and morphological diversity of the L. ankaranensis, L. milanoii-Analafiana group is clinal and explained by geographic distance. These results clarify that L. milanoii is a junior synonym of L. ankaranensis and that the Analafiana forest population belongs to L. ankaranensis, extending its distribution. It further implies that the 'sympatric' zone, the Andrafiamena forest, hosts conspecific individuals with slightly differentiated mtDNA backgrounds, rather than slightly differentiated sympatric species. Lastly, we re-evaluate the IUCN conservation metrics of L. ankaranensis, which continue to qualify as Endangered (EN) under the B1ab(i-v) criteria.

As wolves (Canis lupus) recolonize diverse environments, the extent to which ecological and individual factors shape their spatial behaviour remains insufficiently understood. Italy, where wolf recovery began earlier than elsewhere in Europe and spans highly heterogeneous landscapes, offers an opportunity to explore patterns relevant to future European scenarios. We analysed variation in home range size and movement patterns using GPS data from 19 wolves monitored between 2019 and 2025 across five study areas representing a broad environmental gradient. Continuous-time movement modelling was used to estimate home range size and distance travelled. Home range size varied markedly among areas, with annual averages for pack-member wolves ranging from 35 to 185 km2. The smallest ranges occurred where wolves had been established for longer and/or where prey availability was higher, suggesting that both intraspecific competition and resource abundance influence space use. Wolves expanded their ranges in winter and in more urbanized landscapes. Pack members consistently had smaller home ranges than floaters, and among floaters, females showed slightly larger ranges than males. Distance travelled also differed significantly across areas (local averages 25-53 km/day) but showed no significant association with individual traits. Three dispersal events were documented, two of which resulted in successful pair formation and subsequent reproduction. Overall, our results provide a broad overview of wolf spatial behaviour in contemporary Italian landscapes and offer insights relevant to the management of wolf populations across Europe.

Classic socio-ecological theory predicts that males and females experience different sources and mechanisms of social competition. Whether these differences translate into sex-specific structural properties of dominance hierarchies remains unclear. Here, we compiled 156 dominance interaction matrices from 80 published studies and extracted three commonly used metrics - hierarchy steepness, linearity and the directional consistency index - to investigate the structural characteristics of male and female dominance hierarchies across primates. All three metrics were strongly affected by methodological and demographic variables. Steepness increased with the number of recorded interactions and group size, linearity decreased as matrices became sparser, and directional consistency declined with increasing numbers of interactions. Steepness covaried positively with both linearity and directional consistency, indicating that groups with steeper hierarchies also exhibited more linear and more directionally consistent relationships. We found no sex differences in steepness, linearity or directional consistency. These results suggest that current metrics primarily reflect variation in the sampling effort and the rate of interaction of the recorded behaviour and appear therefore not to capture potential sex differences in the forms of competition. Our findings highlight the need for alternative measures of power asymmetries that are less confounded by sampling effort and demographic variation to better understand how competition and conflict are structured across primate societies.

Zoological gardens strive to prioritize excellent animal care and adhere to the highest standards of animal welfare, ensuring that the conditions of animals` social and physical environment are as close as possible to those in the wild. This study investigates the effects of group split and relocation to the new enclosure on the behaviour of western lowland gorillas (Gorilla gorilla gorilla) (N = 7) living in Prague Zoo, Czech Republic. We conducted over 289 hours of behavioural observations focusing on daily activities, social interactions, and behaviours that serve as potential stress and welfare indicators. The group split led to establishment of two groups in two separate enclosures; the old enclosure consisted of a "bachelor group" (i.e. three males) and a new enclosure consisted of a mixed-sex group (i.e. three females and one juvenile male). The behavioural comparisons across different study periods were conducted using linear mixed models (LMMs). The changes led to an increase in time spent moving, feeding, being in social proximity, and higher rates of approaches among the gorillas, as well as to a decrease in rates of self-directed and "undesirable" behaviors. Our findings indicate that the gorillas effectively adapted to the changes, most likely by relying on social support, to navigate new conditions. This study contributes to our understanding of how socio-cognitively complex species cope with necessary alterations in captive animal care programs. Furthermore, these observations may inform strategies to enhance the welfare of zoo-housed animals and to improve their captive care.

Predation is a major selective force shaping lemur behaviour and population dynamics, yet direct observations remain rare, particularly for small nocturnal species. Here, we document predation on mouse lemurs (Microcebus spp., Cheirogaleidae) in Andohahela National Park, south-eastern Madagascar, and using complementary evidence from radio-telemetry and owl pellet analyses. Two radio-collared individuals were confirmed as prey of endemic snakes, Ithycyphus oursi and Madagascarophis meridionalis (both Pseudoxyrhophiidae), representing the first documented records of Microcebus predation by these species. Examination of owl pellets and prey remains from three sites and from three owl species revealed a single predation event by Asio madagascariensis (Strigidae), likely involving M. tanosi, no evidence of mouse lemur predation by Tyto alba (Tytonidae) despite high local prey availability, and a single and first predation record from Athene superciliaris (Strigidae). Material from A. superciliaris roosts was otherwise dominated by invertebrates, indicating that primate predation is likely opportunistic. Together, these findings expand the known predator guild of mouse lemurs and suggest that snake predation is rarely detected, and its contribution to mouse lemur mortality and population dynamics is likely underestimated. Our findings demonstrate how combining behavioural field observations with dietary evidence can uncover otherwise undetected predation events and clarify predator prey relationships in nocturnal primates.

Brydes whales (Balaenoptera edeni edeni) are nationally protected in China, and the waters around Weizhou Island in the Beibu Gulf support one of the countrys few regularly observed coastal groups. However, acoustic data for this population remain limited, and the potential effects of local vessel noise are poorly described. We conducted 16 vessel-based surveys around Weizhou Island and adjacent waters in January 2024 using a low-disturbance sailboat platform and passive acoustic recorders, with concurrent visual observations where possible. We identified 734 low-frequency signals classified as putative Brydes whale vocalizations and quantified their temporal and spectral parameters. Call duration was significantly negatively correlated with maximum frequency and center frequency, but not with minimum frequency or bandwidth. Comparisons with published records indicate that the recorded signals are most similar to vocalizations previously reported from juvenile Brydes whales or mother-calf pairs, although individual source attribution could not be confirmed. Speedboat passage significantly increased root-mean-square sound pressure levels, and the dominant noise band overlapped the frequency range of the recorded Brydes whale signals, indicating potential for acoustic masking. These results expand the bioacoustic baseline for Brydes whales in Chinese coastal waters and provide evidence relevant to the management of vessel activity and whale-watching tourism around Weizhou Island.

The vast majority of birds are diurnal and concentrate their vocal activity during daylight hours. However, some diurnal birds can also be vocally active at night, although the functions of this phenomenon remain poorly understood. We conducted playback experiments in the Warta Landscape Park (central Poland) to determine whether nocturnal singing by diurnal birds serves breeding-related functions by analysing responses to playback of songs from unfamiliar conspecific males. The sedge warbler (Acrocephalus schoenobaenus) was selected as the focal species because it exhibits relatively high levels of nocturnal song activity, while nine additional diurnal species detected near focal sedge warbler territories were included to explore whether responsiveness to nocturnal conspecific song extended across a broader taxonomic range. Playback experiments were conducted during the early and late stages of the breeding season and during the early and late parts of the nautical night. Out of 10 species tested, three responded vocally: sedge warbler, Savi s warbler (Locustella luscinioides), and common snipe (Gallinago gallinago). Sedge warblers did not modify song rate and song duration but increased flight activity after nocturnal playback. Savi s warblers and common snipes produced more vocalisations after playback than before in May, a pattern consistent with territorial defence function. General nocturnal vocal activity was higher at the beginning of the season, suggesting that birds motivation to establish territories and form pairs can extend into the night, providing additional benefits. Moreover, the probability of singing by the sedge warbler was higher in the latter part of the night. Our study demonstrates that nocturnal stimulation of foreign male playback of diurnal birds can elicit vocal responses from conspecifics, suggesting that nocturnal singing can occur in the absence of obvious artificial light pollution, but in the case of some species and environmental conditions, it may contribute to nocturnal social communication, especially in the early breeding season.

Approaching conspecifics is a fundamental component of social interactions. Such decisions are typically investigated through the study of social relationships (e.g., kinship, dominance, affiliation) and their impact. In the framework, the role of social affordances, i.e., interaction opportunities arising from the immediate social environment, remains poorly understood. We investigated how context-dependent factors shape approach decisions in wild crested macaques (Macaca nigra) in North Sulawesi, Indonesia. From September 2024 to May 2025, we collected data on 27 adults (20 females, 7 males) using focal observations and an approach-specific protocol recording neighbours identities and behaviours at the initiation of close-proximity approaches (<1 body length). We first compared data collected during approaches with focal data, then conducted within-approach analyses contrasting approached partners and bystanders. We further tested the effects of behavioural state, subgroup size and composition, including infant presence. Bayesian models revealed that affiliative, neutral, and grooming behaviours were more frequent in approach contexts than in general observations. Within approaches, individuals avoided congeners already engaged in grooming and approached individuals in neutral or affiliative states (e.g., lip-smacking, embracing). Macaques globally approached more isolated individuals or dyads. When multiple subgroups were present (i.e., multiple choices), the likelihood of being approached increased with subgroup size and was higher in subgroups containing infants. These findings indicate that macaques use behavioural cues and local social density as affordance signals, adjusting approach decisions to maximize interaction opportunities and navigate complex social environments.

Humans are widely regarded as unusually slow to develop, exhibiting prolonged childhood and extended dependence on caregivers. However, this view is based primarily on comparisons with other primates, leaving unresolved whether humans remain distinctive within the broader diversity of mammals. We addressed this question by situating human development in a comparative framework using gestation length, weaning age, and age at sexual maturity for both sexes across 462 mammalian species representing 25 orders. Each trait was examined both as an absolute value and as a proportion of the longest verified captive lifespan. In absolute terms, human developmental traits fell within the upper range of mammalian variation. When expressed relative to lifespan, however, gestation shifted toward the lower end of the distribution, whereas weaning age and sexual maturity occupied intermediate positions, indicating that human developmental timing largely follows general mammalian scaling patterns rather than representing a pronounced outlier. These findings suggest that key features of human dependency are better understood as extensions of broader evolutionary trends than as uniquely human life-history characteristics.

For many group living species, vocal signals are a vital form of communication needed to coordinate social behaviours. Investigating these processes first requires a detailed description of the species vocal repertoire. White-nosed coatis (Nasua narica), which forage and move together in forest habitats, are thought to rely on vocalisations to coordinate movements and maintain group cohesion. However, quantitative studies of their vocal repertoire are lacking. We examined the vocal repertoire of white-nosed coatis from wild populations in Panama and Arizona, USA, to gain a more comprehensive view of their calling behaviours. By combining traditional acoustic analyses with an unsupervised approach based on spectrogram structure, we characterised the diversity of calls in this species and described the temporal and structural features of their vocalisations. We identified 19 call types, with some of these calls emitted in multi-syllable call sequences or in fast succession. We found variability in call rates among group members, which may be driven by differences in social status within the group. In addition, our results indicate that white-nosed coatis likely have individually-recognisable vocalisations. This study provides a foundational description of the white-nosed coati vocal repertoire, laying the groundwork for future research on vocal communication in this species.

Chorus howls play a central role in wolves' (Canis lupus) social communication, shaping both intra- and inter-pack interactions. While synchrony has been shown to influence perceived threat from outgroups in birds and humans, whether the synchrony of an outside pack's chorus howl plays a role in wolf pack behaviour remains unclear. This study investigated behavioural responses of 19 zoo-housed wolf packs to playbacks of synchronous and asynchronous chorus howls from unfamiliar packs, in comparison to a dove control call. We hypothesized that packs would show stronger intra-pack cohesion and greater spatial withdrawal in response to more synchronous, and thus cohesive - and potentially more formidable - sounding vocalisations. Alternatively, asynchronous howls might elicit stronger responses due to their ambiguity and the "Beau Geste" effect (where asynchronous signals make it harder to infer the number of callers). Synchronous howls resulted in more distance from the sound source and less activity compared with asynchronous howls. This could be interpreted as a more cautious response towards a perceived threat. However, since there was no effect on pack-directed behaviour and no difference between the synchronous and control sound, lower interest or motivation to engage with the stimulus cannot be excluded. In contrast, wolves spent more time near the sound-source after hearing asynchronous howls, approached it more, and oriented towards it for longer compared to synchronous playbacks, a pattern consistent with threat-investigation. In how far these reactions result from a more difficult localisation of synchronous howls or more ambiguity in pack size in the asynchronous playbacks, as well as how this translates to wild wolf behaviour, remains to be investigated.

Non-human primates are crucial animal models in biomedical research including neurosciences. Legal frameworks ensure that participation of monkeys in biomedical research follows an ethical assessment, driven by specific guidelines for ensuring animal well-being. Those guidelines promote continued refinement of practices to improve animal wellbeing, yet quantitative data to inform such refinements is limited. In this context, we investigated the impact of modifications to the housing that created either navigational complexity or visual access to neighbouring animals on the behaviour of rhesus macaques. Then we quantified the value of different enrichment programmes based on manipulable objects. We showed that making navigation within a modular housing more complex, or adding transparent separations between housing units is associated with significant reduction of severe aggression, stereotypical behaviours and a significant increase of appeasing behaviours. We also observed a positive effect of manipulable objects with an increase in the expression of appeasing behaviours and a decrease in abnormal or stereotypical behaviours. Finally, our study revealed significant inter-individual variability that was not explained by sex, age, social network size or social status. Overall, our results point to the importance and benefits of environmental changes and enrichment protocols, and of the critical need for personalised enrichment programmes.

Many social insects, such as ants, change their behavior stereotypically from nursing to foraging as they age, a concept known as temporal polyethism. Biogenic amines are associated with these specific behaviors, but much about them remains poorly understood in these animals. Many aminergic systems lack anatomical characterization, and little is known about the expression of genes required for the aforementioned behavioral phenotypes. Here, we studied Pogonomyrmex barbatus brains and identified the neurons that produce two relevant amines, dopamine and octopamine, by detecting their synthesis enzymes, tyrosine hydroxylase and tyramine beta-hydroxylase, and their transcripts. We also compared the expression of both genes in young nurses and mature foragers by measuring the fluorescence intensity. Dopaminergic and octopaminergic neurons are predominantly located in the protocerebrum and in the subesophageal zone. Neurons that produce dopamine are also present in the optic lobes, whereas octopamine-producing ones show clusters in the antennal lobes. Both genes are downregulated as the organism ages. A reduction in the expression of these genes might be correlated with the mature workers increased propensity to perform extranidal tasks, such as foraging.

One challenge of reintroduction programs is ensuring that captive individuals are prepared for life in the wild, including having the ability to forage on wild resources. Foraging proficiency can be especially challenging to foster in species with a wide dietary breadth or reliance on ephemerally available resources. A relatively unexplored obstacle to release readiness may be an animals hesitancy to approach or consume unfamiliar foods, i.e. their neophobia and dietary conservatism. In this study, we examined the responses of a conservation breeding population of the extinct-in-the-wild alal[a] (Corvus hawaiiensis), a dietary generalist, to novel and familiar native and non-native fruits. Birds were similarly willing to contact both novel and familiar fruits, suggesting little aversion to the presence and appearance of novel food items. However, birds showed a clear preference for contacting non-native fruits over native fruits, regardless of familiarity. Additionally, while birds were willing to contact novel items, they were less likely to consume novel native fruits, suggesting that dietary conservatism may limit incorporation of these resources in the wild. Finally, juvenile birds were less likely to approach the testing setup and contact items, suggesting that neophobia may constrain their exploration of novel environments and resources. We discuss the implications of alal[a] preferences for non-native foods and reluctance to consume novel native items for pre-release preparations and the goal of wild species recovery.

EU has reclassified the sika deer (Cervus nippon) as an undesirable invasive species based on reports that hybridization with the indigenous red deer (C. elaphus) may produce fertile offspring. Since sika-derived DNA previosuly introduced into the red deer population (introgression) cannot be removed, the crucial question is whether new (F1) hybridisation occur. To address this, we analysed the chromosomes in 56 sika and 22 red deer. All red deer had a chromosome number 2n=68. In contrast, the chromosome number in sika ranged from 64 to 67, due to the variable presence of two sika-specific Robertsonian translocations (ROB1,ROB2). In the free-ranging sika population in Jutland, >90% of the sika deer were homozygote for at least one of these ROBs, excluding that they could be F1-hybrids. Moreover, ROB2 was in Hardy-Weinberg equilibrium, further supporting the absence of gene flow between the two species. In contrast, ROB1 was in Hardy-Weinberg disequilibrium, suggesting negative fitness of heterozygotes, including potential F1-hybrids. In Jaegersborg Deer Park, the eight examined sika deer had the same genotype (absence of ROB1, homozygosity of ROB2), supporting that it is a founder population which may have been isolated for [~]100 years. Again, none of these can be F1-hybrids due to the homozygosity of ROB2. We conclude that F1-hybridisation between sika and red deer either does not occur or occur very rarely in Denmark. The study establish the Danish sika-populations as unique models for adressing important biological questions: What underlies the absence of hybridisation? Why are ROBs frequent in sika deer but not in the closely related red deer? How fast do new species/subspecies develop in isolated founder populations? Which factors determine, that some ROBs have little heterozygous effects, whereas others are selected against, with implications for the role of ROBs as genetic barriers promoting speciation, and for fertility problems in some human ROB carriers.

Understanding the spatial patterns of biodiversity and their environmental determinants is fundamental to ecology and conservation, particularly in mountainous transitional zones where species assemblages can shift rapidly. Amphibians, as highly sensitive organisms, are excellent indicators of environmental change, yet quantitative assessments of their diversity in the Huangshan Mountains-a biodiversity hotspot at the junction of the Palaearctic and Oriental realms in southern Anhui, China-remain limited. We conducted systematic line-transect surveys across 200 grids (5 km x 5 km) during the spring and autumn of 2023 and 2024, recording a total of 10,342 individuals belonging to 23 species, 20 genera, 9 families, and 2 orders. Three species (Fejervarya multistriata, Microhyla fissipes, and Bufo gargarizans) were identified as dominant, and two nationally protected species (Hoplobatrachus chinensis and Andrias davidianus) were detected. Inter-annual alpha diversity did not differ significantly, but pronounced seasonal variation was observed, with spring supporting higher Shannon-Wiener and Pielou evenness than autumn in both years. Using generalized additive models and model averaging, we found that annual precipitation and the normalized difference vegetation index (NDVI) were consistently the strongest positive predictors of Shannon-Wiener diversity, Simpson dominance, and species richness, while species richness also declined significantly with increasing human footprint. Total beta diversity (Soerensen dissimilarity) was very high (0.981) and overwhelmingly driven by species turnover (97.86%) rather than nestedness. Partial Mantel tests and distance-based redundancy analysis further revealed that environmental distances-particularly annual precipitation-significantly shaped overall beta diversity and its turnover component after accounting for geographic distance. These results highlight the predominant role of climatic and vegetation gradients in structuring amphibian assemblages in this subtropical mountainous region, providing baseline data to inform local conservation strategies.

Nonhuman primates (NHP) are crucial models of human health and disease and offer the opportunity to bridge the gap between basic research and the clinic. Given the ethical considerations, high costs, and logistical constraints associated with NHP studies, careful planning is essential to maximize scientific rigor while minimizing animal use. Statistical power calculations, to determine optimal sample size, require knowing the expected number of animals at each timepoint, a challenge for longitudinal studies that must account for natural deaths or disease during a study. Here, we leveraged data from a recent study detailing NHP life- and healthspans to develop a web-based NHP study design tool, available at midas.wakehealth.edu. For 11 NHP species relevant to biomedical research, users can provide study details to generate estimates of sample counts based on natural healthspan trajectories. We envision this tool being used by colony managers and investigators for both study design and monitoring colony health over the course of a study.