Biophysical characterization of novel biomarkers and bioaffinity reagents (NanoMIPs): on the road for a low-cost diagnostic test for intestinal schistosomiasis
Isopo, F.; Stephen, A. N.; Boumis, G.; Giorgi, A.; Eberini, I.; Reddy, S. M.; Miele, A. E.
Show abstract
Schistosoma mansoni is a vector-borne intestinal parasite, endemic in [~]70 tropical countries. Most of the World Health Organization interventions are based on mass drug administration (MDA) and sanitation. Since the parasite does not induce permanent immunity, reinfection rate is high, inducing scheduling of several MDA campaigns. To follow up after treatment and avoid blind MDA, providing a new rapid and cheap diagnostic test is needed, since the most used method is fifty years-old, lacks sensitivity and efficiency. Venom Allergen-Like proteins (SmVALs) have been previously identified as secreted/excreted proteins and potential biomarkers. Here we present the biophysical characterization of SmVAL11 and SmVAL13, their recognition by both specific antibodies and molecularly imprinted polymers (nanoMIPs), an easy-to-standardize alternative. The specific antibodies showed no cross-reactivity, while initial characterization of the nanoMIPs indicates a promising level of selectivity, establishing them as a reliable, cost-effective alternative. Therefore, our results are promising for the future development of a new cheap diagnostic test of intestinal schistosomiasis.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Scoping review of the applications of peptide microarrays on the fight against human infections. 92%
- Identification of French Guiana anopheline mosquitoes by MALDI-TOF MS profiling using protein signatures from two body parts. 92%
- Impact of Sugar-Based Baits on Midgut Microbiome Composition in Aedes Mosquitoes: Implications for Vector Control 92%
Similar papers in this journal
- Shrimp Hemocyanin elicits a potent humoral response in mammals and is favorable to hapten conjugation 93%
- Molecular malaria surveillance using a novel protocol for extraction and analysis of nucleic acids retained on used rapid diagnostic tests 92%
- Lupin (Lupinus sp.) seeds exert anthelmintic activity associated with their alkaloid content 91%
Similar papers in this journal
- Rapid identification of Biomphalaria spp. and diagnosis of Schistosoma mansoni infestation using MALDI-TOF mass spectrometry 94%
- MALDI-TOF : A new tool for the identification of Schistosoma cercariae and detection of hybrids 93%
- Proteomic fingerprint identification of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library 93%
Similar papers in this journal
- Synthetic and plant-derived multivalent galactans as modulators of cancer-associated galectins-3 and -9 91%
- Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive 89%
- Antifungal therapeutic potential of Candida albicans Fun30: screening and validation of novel inhibitors against Candida albicans 89%
Similar papers in this journal
- Comparative analyses of SARS-CoV-2 binding (IgG, IgM, IgA) and neutralizing antibodies from human serum samples 90%
- Peptide fibrils as vaccine: a proof of concept 90%
- A High-throughput Multi-Species Platform Using Biolayer Interferometry Immunosorbent Assay (BLI-ISA) as an Alternative to Indirect ELISA for Vaccine Development 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.