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Protocol for assessing lysosomal ion channel function in mammalian cells using lysosomal patch-clamp technique

Wang, Y.; Jan, L. Y.

2026-06-02 cell biology
10.64898/2026.06.01.729450 bioRxiv
Show abstract

This protocol describes manual whole-endolysosome patch-clamp recordings from pharmacologically or genetically enlarged endolysosomes in cultured mammalian cells. Steps include vesicle enlargement, fabrication and fire-polishing of high-resistance pipettes, mechanical dissection and isolation of enlarged vesicles, giga-seal formation, and configuration to whole-endolysosome modes. For complete details on the use and execution of this protocol, please refer to Wang et al1.

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