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Intracortical vessel density mapping at mesoscale reveals regionally specific angioarchitecture in the human brain

Gulban, O. F.; Wagstyl, K.; Huber, R.; Pizzuti, A.; Bollmann, S.; Roebroeck, A.; Goebel, R.; Kay, K.

2026-05-05 neuroscience
10.64898/2026.05.01.722251 bioRxiv
Show abstract

The metabolic demands of the human brain are met by a complex vascular architecture, yet our characterization of this network remains incomplete. While we have mapped the macroscopic vessels on the brains surface and the microscopic capillaries within small tissue samples, the mesoscopic scale consisting of the penetrating vessels that plunge through cortex remains an anatomical terra incognita. Mapping the interface between the macroscopic and microscopic scales is essential to understanding the critical vascular supply that sustains brain health. Here, we leveraged the BigBrain dataset and developed custom detection and tracing algorithms to reveal a whole-cortex record of the mesoscopic vascular network. We find that vascular density is not uniform across the cortex, but is a heterogeneous landscape that shows clear relationships to traditional areal boundaries. While based on a single human specimen, our results constitute a reference for human mesoscopic angioarchitecture and demonstrates the power of repurposing high-resolution histological atlases. Ultimately, this work lays the groundwork for validating recently developed in vivo MRI techniques for imaging the human cerebrovascular system at mesoscale.

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