Aged-black garlic (Abg10+) supplementation effect in blood lipoprotein profile in Grade I Hypertensive subjects. A randomized, triple-blind controlled trial.

Aged black garlic (ABG) is rich in organosulfur compounds such as Sallylcysteine (SAC) and may influence lipid metabolism, although evidence from controlled trials remains limited. The aim of this study was to evaluate the impact of a low-dose SAC-optimized ABG on blood lipid profiles in Grade I hypertensive individuals. A randomized, triple-blind, placebo-controlled trial was conducted with 75 Grade I hypertensive participants receiving either 250 mg of ABG or a placebo daily for 12 weeks. Plasma lipoproteins and subclass composition were quantified by NMR spectroscopy, and metabolic clusters were explored using kmeans and PLSDA. ABG supplementation led to a significant reduction in the total number of particles and HDL particles. Detailed analysis of XXL-VLDL particles showed a significant decrease in the percentage of both free and esterified cholesterol, alongside an increase in triglyceride percentage. Conversely, large HDL particles exhibited a beneficial remodeling characterized by an increase in phospholipid content and a decrease in triglyceride percentage. Furthermore, cluster analysis demonstrated that participants with a more adverse baseline metabolic profile experienced a significant reduction in total triglycerides and VLDL-lipid content after ABG intake. These results suggest that low-dose ABG supplementation induces specific qualitative improvements in lipoprotein subclasses, particularly enhancing HDL functionality markers and reducing the cholesterol load in large VLDL particles, which may provide cardiovascular benefits in hypertensive individuals with metabolic impairment