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High prevalence of loss of Y chromosome in the spermatozoa of young cancer survivors

Axelsson, J.; Bruhn-Olszewska, B.; Sarkysian, D.; Markljung, E.; Horbacz, M.; Pla, I.; Sanchez, A.; Nenonen, H.; Elenkov, A.; Dumanski, J. P.; Giwercman, A.

2026-03-23 genetic and genomic medicine
10.64898/2026.03.20.26348822 medRxiv
Show abstract

Cancer-related genomic instability (GI) may cause genetic alterations in spermatozoa, implying health issues not only in cancer survivors, but also in their children [1, 2]. We therefore studied Loss of Y chromosome (LOY), considered as hallmark of GI [3-15], in spermatozoa and blood from survivors of childhood and testicular cancer (CC, TC), and controls (CTRL). We found that LOY was statistically significantly more frequent in spermatozoa from cancer survivors than in controls (Odds Ratio [OR]=2.2 for CC vs. CTRL and OR=2.4 for TC vs. CTRL). Furthermore, LOY was about an order of magnitude more prevalent in spermatozoa than in blood among 18-53-year-old males within all cohorts. Our findings suggest that LOY in spermatozoa might be a clinically useful marker of GI, reduced fertility and disease predisposition in males. Introducing LOY in spermatozoa as a biomarker opens a new research avenue into disease prevention and the causes and consequences of LOY.

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