The SARS-CoV-2 nonstructural protein 15 collapses the host cytoskeleton by interacting with the host keratin type II cytoskeletal 1
Tam, D.; Monjaras-Avila, C.; Adomat, H.; Bach, H.
Show abstract
COVID-19, the deadliest recorded pandemic of the 21st century, was caused by a novel coronavirus, SARS-CoV-2. To explore why this coronavirus was significantly more pathogenic than its predecessors, more information is needed about the functions of the viral proteins that contributed to this uptick in virulence. This study explored how one of these viral proteins, the nonstructural protein 15 (NSP15), interacts with the host. Our findings revealed a novel protein-protein interaction between NSP15 and the host keratin type II cytoskeletal 1 in Vero E6 cells, as determined by mass spectrometry, and subsequently validated by immunoprecipitation. We observed that when NSP15 was delivered into Vero E6 cells, the fibrous network of keratin intermediate filaments was disrupted, and the nuclear structure was lost. This disruption of the keratin cytoskeleton was then shown to cause a statistically significant reduction in cell viability of Vero E6 cells. These results indicate that the presence of NSP15 in host cells leads to a collapse of the keratin cytoskeleton. This cytoskeletal collapse could be a mechanism the virus employs to escape the infected host cell via cell lysis, once significant amounts of viral protein and progeny have accumulated.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- DNA damage response signaling is crucial for effective Chikungunya virus replication 95%
- Stearoyl coenzyme A desaturase 1 (SCD1) regulates foot-and-mouth disease virus replication by modulating host cell lipid metabolism and 2C-mediated replication complex formation 94%
- Modulation of Voltage-Gated Sodium Channel (VGSC) Activity in Human Dorsal Root Ganglion (DRG) Neurons by Herpesvirus Quiescent Infection 94%
Similar papers in this journal
- Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire 95%
- Difference in TMPRSS2 usage by Delta and Omicron variants of SARS-CoV-2: Implication for a sudden increase among children 94%
- The chicken chorioallantoic membrane model for isolation of CRISPR/cas9-based HSV-1 mutant expressing tumor suppressor p53 94%
Similar papers in this journal
Similar papers in this journal
- MK2a inhibitor CMPD1 abrogates chikungunya virus infection by modulating actin remodeling pathway 94%
- An Arginine-Rich Motif in the ORF2 Capsid Protein Regulates the Hepatitis E Virus Lifecycle and Interactions with the Host Cell 94%
- CVB3 VP1 interacts with MAT1 to inhibit cell proliferation by interfering with Cdk-activating kinase complex activity in CVB3-induced acute pancreatitis 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.