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Plasma plasmalogen levels and risk of lymph node positive breast cancer

Yavari, M.; Brantley, K. D.; Carmona, A.; Sabatier, M.; Liang, Y.; Monge-Lorenzo, J.; Torpey, J.; Szylo, K. J.; Flores, M.; Palma, M.; Fraser, C. S.; Chaufan, M.; Kaur, M.; Hendrianto, L.; Henry, W.; Hui, S. T.; Rodriguez, R.; Willett, W. C.; Avila-Pacheco, J.; Clish, C. B.; Eliassen, H. A.; Zeleznik, O. A.; Ubellacker, J.

2025-12-15 cancer biology
10.64898/2025.12.11.693510 bioRxiv
Show abstract

Lymph node involvement is a key predictor of poor breast cancer prognosis. Systemic lipid alterations can contribute to cancer cell survival in lymph nodes, but their relevance in humans remains unclear. Here, we combine human epidemiologic analyses from the Nurses Health Study 2 and complementary mechanistic studies in mouse models to investigate how systemic lipid profiles relate to lymph node positive breast cancer. We show that in pre-diagnostic human plasma (n=511), lower levels of phosphatidylethanolamine (PE) and phosphatidylcholine (PC)-enriched plasmalogens are associated with increased risk of lymph node positivity, with stronger associations in samples collected closer to diagnosis. Consistent with the human data, lower levels of PE and PC-enriched plasma plasmalogens are found in mice with nodal involvement. Furthermore, in mice, dietary PUFA depletion reduces lipid oxidation in breast cancer cells in lymph nodes and promotes their survival and metastatic spread. These findings suggest that reduced levels of PE and PC-plasmalogens and decreased PUFA availability creates a lipid environment that enables breast cancer lymph node involvement.

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