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Analytical meditation improves physiological well-being in expert practitioners: a study on central and peripheral neurophysiological correlates

Callara, A. L.; Azarabad, M. H.; Sebastiani, L.; Sherab, N.; Khechok, J.; Tsering, J.; Thakchoe, J.; Soepa, J.; Vanello, N.; Scilingo, E. P.; Greco, A.; Neri, B.

2025-12-11 neuroscience
10.64898/2025.12.08.692357 bioRxiv
Show abstract

Meditation has been long associated with improvements in mental well-being, emotional regulation, and attentional control. Yet, the diversity of meditative techniques and participant expertise has hindered the systematic identification of their neurophysiological correlates supporting these benefits. To address this challenge, we investigated the neurophysiological signatures of concentrative and analytical meditation in 35 experienced Tibetan monk practitioners. EEG, ECG, EDA, and respiration were simultaneously recorded during Concentrative, Loving-Kindness and Emptiness meditations. Linear-mixed-models revealed significant modulations in autonomic and cortical activity across meditations. Peripheral indices indicated enhanced parasympathetic tone, decreases respiratory rate, and gradual increase in EDA - reflecting a state of relaxed-alertness with concurrent vagal engagement and sustained sympathetic arousal. EEG analyses supported this state showing elevated gamma-band power during analytical meditations. These findings suggest that advanced meditative states foster an adaptive integration of autonomic and cortical responses, supporting the emergence of relaxed-vigilance - a psychophysiological condition associated with well-being and cognitive flexibility.

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