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Inferring blood volume collected on plasma-separating dried blood spots using mass and image analysis

2025-12-02 oncology Title + abstract only
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BackgroundPlasma-separating dried blood spots (psDBS) allow measurement of cancer biomarkers such as cell-free DNA concentration, enabling distributed sample collection, as well as ambient storage and shipment. However, unlike volumetric blood tube collections, blood volume collected using psDBS can vary between individuals. We evaluated whether analysis of psDBS mass, area, and color can be used to infer blood volume, and enable downstream measurement of circulating analyte concentrations. Met...

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