Quantitative Detection of Cell Activity by Measuring the Fluctuation of Intracellular Motility
Sakuma, M.; Kondo, Y.; Higuchi, H.
Show abstract
The measurement of cell activity changes during damage is important to understand the process of cell death and evaluate the effect of medicines. To evaluate cell activity generally, we extended the method of intensity fluctuation in which intensity change in the pixel induced by the movement of organelles was calculated. Cancer, endothelial and iPS cells were damaged by reactive oxygen species (ROS) generated by a fluorescent dye (IR700), hydrogen peroxide, and ultraviolet light. The intensity fluctuation in damaged cells gradually decreased independent of the kind of cell, indicating that the decrease in the fluctuation is a general phenomenon in damaged cells. The rupture of vesicles and mitochondria in the cells were observed upon ROS production. The motility of purified kinesin and dynein which transport vesicles and organelles was inhibited by ROS. These suggest that ROS and cytotoxic molecules spreading from ruptured organelles contribute to the reduction in cell activity which brings about the decrease in the motility and intensity fluctuation of organelles driven by kinesin and dynein.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Two new coral fluorescent proteins of distinct colorsfor sharp visualization of cell-cycle progression 93%
- Folding latency of fluorescent proteins affects the mitochondrial localization of fusion protein 93%
- Knock-in of labeled proteins into 5'UTR enables highly efficient generation of stable cell lines 92%
Similar papers in this journal
Similar papers in this journal
- Chromosome counting in the mouse zygote using low-invasive super-resolution live-cell imaging 93%
- Partial exogastrulation due to apical-basal polarity of F-actin distribution disruption in sea urchin embryo by omeprazole 90%
- Possibilities of skin coat color-dependent risks and risk factors of squamous cell carcinoma and deafness of domestic cats inferred via RNA-seq data 90%
Similar papers in this journal
Similar papers in this journal
- Non-destructive in situ monitoring of structural changes of 3D tumor spheroids during the formation, migration, and fusion process 94%
- Rapid mechanical stimulation of inner-ear hair cells by photonic pressure 92%
- Automated workflow for the cell cycle analysis of non-adherent and adherent cells using a machine learning approach 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.