Effect of temperature and agitation rate variation on the growth of NS0 cell line in monoclonal antibody production
Shetty, S.
Show abstract
The quality and yield of the monoclonal antibodies produced in a cGMP environment is heavily influenced by the bioprocess-related parameters which impact the cell growth and metabolism of the mammalian cell cultures. This research report describes a study conducted to examine the effects of varying temperature and RPM set points on viable cell density and viability of NS0 cultures. All cultures were grown in 250 mL shake flasks (working vol. 100 mL). To separately analyze the effects of temperature and agitation rate on NS0 cell metabolism, flask stage cultures were evaluated in triplicates at two cultivation temperatures (36 {degrees}C and 38 {degrees}C) and two agitation rates (120 RPM and 160 RPM) while controls were maintained at 37 {degrees}C and 140 RPM for both the conditions using an incubator. Flasks were sampled every 24 h and analyzed for viable cell density and % viability. Additional data was collected on pH, pO2, pCO2, osmolality, glucose, lactate, glutamate and glutamine levels in the culture. It was observed that variations in temperature has the greatest effect on viable cell density and viability and varying agitation rates had minimal effect on growth of cultures. A temperature set point of 38 {degrees}C is detrimental to the culture growth. The control set points proved to be optimal for this process.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Enhancing pigment production by a chromogenic bacterium (Exiguobacterium aurantiacum) using tomato waste extract: A Statistical approach 96%
- Production, purification and evaluation of biodegrading potential of PHB depolymerase of Stenotrophomonas sp. RZS 7 95%
- Seed biopriming with P- and K-solubilizing <em>Enterobacter hormaechei</em> sp. improves the early vegetative growth and the P and K uptake of okra (<em>Abelmoschus esculentus</em>) seedling 94%
Similar papers in this journal
- Characterizing the effect of impeller design in plant cell fermentations using CFD modeling 96%
- An open-source computational tool for measuring bacterial biofilm morphology and growth kinetics upon one-sided exposure to an antimicrobial source. 94%
- High-throughput bacterial aggregation analysis in droplets 94%
Similar papers in this journal
- The VVBlue assay: a plate-readable, dye exclusion-based cell viability assay for the toxicological testing of chemicals 93%
- Oxidative stress induced by tBHP in human normal colon cells by label free Raman spectroscopy and imaging.The protective role of natural antioxidants in the form of β-carotene 93%
- Microbial-induced calcium carbonate precipitation: An experimental toolbox for in situ and real-time investigation of micro-scale pH evolution 91%
Similar papers in this journal
- Debottlenecking and reformulating feed media for improved CHO cell growth and titer by data-driven and model-guided analyses 94%
- Computational evaluation of light propagation in cylindrical bioreactors for optogenetic mammalian cell cultures 92%
- Bioprinting transgenic plant cells for production of a recombinant biodefense agent 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.