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Effects of Shift-Work on the Carotid Artery and Cerebral Blood Flow of SHR Rats and WKY Rats

Wang, Y.; Zhang, T.; Zhang, Y.; Yu, Y.; Bai, F.; Zhang, H.; Chi, Y.; Gao, S.

2019-08-20 pathology
10.1101/740068 bioRxiv
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ObjectiveThe objective was to investigate the effects of shift-work (SW) on the carotid arteries.\n\nMethodsThis study used two inverted photoperiods (inverted light:dark [ILD]16:8 and ILD12:12) to create the SW model. We recorded the rhythm and performed serological tests, carotid ultrasound, magnetic resonance imaging, and carotid biopsy.\n\nResultsSW induced elevated blood pressure and increased angiotensin-II, apolipoprotein E, blood glucose, and triglycerides. SW increased the carotid intima-media thickness. SW led to the development of carotid arterial thrombosis, reduced cerebral blood flow, and increased the number of collagen fibers, expression of angiotensin receptor and low-density lipoprotein receptor in the carotid arteries. SW decreased 3-hydroxy-3-methylglutaryl-CoA reductase and nitric oxide. SW induced the atherosclerotic plaque in the aorta. Multiple results of SHR were worse than WKY rats.\n\nConclusionSW can induce metabolic disorders and elevated blood pressure. SW can cause intima-media thickening of the carotid artery and aorta atherosclerosis. SW may result in carotid arterial thrombosis and affect cerebral blood flow. Hypertension can aggravate the adverse effects of SW.

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