A comparison of DNA stains and staining methods for Agarose Gel Electrophoresis
Hall, A. C.
Show abstract
Nucleic acid stains are necessary for Agarose Gel Electrophoresis (AGE). The commonly used but mutagenic Ethidium Bromide is being usurped by a range of safer but more expensive alternatives. These safe stains vary in cost, sensitivity and the impedance of DNA as it migrates through the gel. Modified protocols developed to reduce cost increase this variability. In this study, five Gel stains (GelRed, GelGreen, SYBR safe, SafeView and EZ-Vision(R)In-Gel Solution) two premixed loading dyes (SafeWhite, EZ-Vision(R)One) and four methods (pre-loading at 100x, pre-loading at 10x, precasting and post-staining) are evaluated for sensitivity and effect on DNA migration. GelRed was found to be the most sensitive while the EZ-Vision(R) dyes and SafeWhite had no discernible effect on DNA migration. Homemade loading dyes were as effective as readymade ones at less than 4% of the price. This method used less than 1% of the dye needed for the manufacturer recommended protocols. Thus, with careful consideration of stain and method, Gel stain expenditure can be reduced by over 99%.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- A low-cost smart system for electrophoresis-based nucleic acids detection at the visible spectrum 94%
- Probing Loop-Mediated Isothermal Amplification (LAMP) targeting two gene-fragments of rose rosette virus 94%
- Multiplex detection of "Candidatus Liberibacter asiaticus" and Spiroplasma citri by qPCR and droplet digital PCR 93%
Similar papers in this journal
- PCR procedures to amplify GC-rich DNA sequences of Mycobacterium bovis 95%
- Microliter spotting and micro-colony observation: a rapid and simple approach for counting bacterial colony-forming units 93%
- Bacterial Zombies And Ghosts: Production Of Inactivated Gram-Positive And Gram-Negative Species With Preserved Cellular Morphology And Cytoplasmic Content 91%
Similar papers in this journal
- Laser Capture Microdissection optimization for high-quality RNA in mouse brain tissue 91%
- Establishing Primary and Stable Cell Lines from Frozen Wing Biopsies for Cellular, Physiological, and Genetic Studies in Bats 91%
- Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells 90%
Similar papers in this journal
- Limitations of qPCR to estimate DNA quantity: AnRFU method to facilitate inter-laboratory comparisonsfor activity level, and general applicability 95%
- RFU derived LRs for activity level assignments usingBayesian Networks 95%
- Levenshtein Distance as a Measure of Accuracy and Precision in Forensic PCR-MPS Methods 94%
Similar papers in this journal
- Development and validation of cost-effective one-step multiplex RT-PCR assay for detecting the SARS-CoV-2 infection using SYBR Green melting curve analysis 94%
- A Paper-based Loop-Mediated Isothermal Amplification (LAMP) Assay for Highly Pathogenic Avian Influenza 94%
- A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.