Reevaluating the Role of Beta2-Microglobulin: New Insights on Selective Vulnerability in ALS Pathology

Amyotrophic lateral sclerosis (ALS) is characterized by the selective loss of motor neurons (MNs). Why these neurons show particular vulnerability in ALS is still debated, and also why certain MNs remain resilient. We investigated the importance of the human leukocyte antigen (HLA) and particularly beta2-microglobulin ({beta}2m) in MN susceptibility to ALS as these molecules have been implicated both in prolonging and shortening disease. RNA-sequencing demonstrated that disease-resistant oculomotor neurons (OMNs) and Onufs MNs had similar {beta}2m and HLA mRNA levels to vulnerable spinal MNs in control tissues. Thus, baseline differences in these transcripts do not explain the differential vulnerabilities of these neurons to disease. Nonetheless, the HLA protein level showed an inverse correlation with spinal MN size, where large MNs, which are lost early in ALS, had low HLA levels. HLA protein levels were also reduced in end-stage ALS patient spinal MNs, while remaining unchanged in OMNs with disease. Spinal MNs uniquely exhibited a significant upregulation of {beta}2m and HLA-C transcripts with disease, likely as a protective compensatory response. Thus, {beta}2m and HLA may relate to vulnerability to ALS. Cross-breeding of {beta}2m knockout mice with SOD1G93A ALS mice resulted in modest transient worsening of functional motor performance, but did not affect life span. There was partial preservation of innervation of particular muscles in SOD1G93A mice lacking {beta}2m, which was insufficient to improve motor behavior. These findings indicate that {beta}2m and HLAs are dynamically regulated in ALS, and may influence MN vulnerability, but they are not major disease modifiers in ALS.