Post-treatment recovery of docetaxel-treated prostate cancer monolayer and spheroid culture
Swanson, S.; McKenna, C. B.; Cheraghi, E.; Osei, E.
Show abstract
In the last half-century, it has become widely recognized that small 3D aggregates of cancer cells called tumor spheroids mimic some aspects of tumor behavior. Cell culture geometry has been shown to influence drug pharmacokinetics, delivery, and resistance. Despite the improved physiological relevance, the laborious nature of spheroids has limited clonogenic measurement and longitudinal observation of post-treatment recovery in docetaxel-treated prostate tumor spheroids. Agent-based modeling can complement spheroid experiments by probing questions of interest that are experimentally inaccessible. Here, we performed proliferation and clonogenic assays in docetaxel-treated PC3 cells cultured in monolayers and spheroids to assess and compare end-of-treatment survival and post-treatment recovery. We observed growth stimulation with no survival benefit in low dose docetaxel-treated monolayer and spheroid culture. However, agent-based modeling suggested that this hormetic effect may have been influenced by the active process of apoptosis. To the best of our knowledge, this is the first clonogenic measurement of docetaxel-treated spheroid culture and longitudinal observation of post-treatment docetaxel-dose dependent effects in prostate cancer cell culture.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Reshaping the Landscape of Locoregional Treatments for Breast Cancer Liver Metastases: A novel, intratumoral, p21-targeted percutaneous therapy increases survival in BALB/c mice inoculated with 4T1 triple negative breast cancer cells in the liver. 95%
- Comprehensive Live-cell Imaging Analysis of Cryptotanshinone and Synergistic Drug-Screening Effects in Various Human and Canine Cancer Cell Lines 93%
- Injectable diblock copolypeptide hydrogel provides platform to maintain high local concentrations of taxol and local tumor control 92%
Similar papers in this journal
- mTOR inhibitors as radiosensitizers in neuroendocrine neoplasms 93%
- Short-term circulating tumor cell dynamics in mouse xenograft models and implications for liquid biopsy. 91%
- A pro-oxidant combination of resveratrol and copper down-regulates hallmarks of cancer and immune checkpoints in patients with advanced oral cancer: Results of an exploratory study (RESCU 004) 91%
Similar papers in this journal
- Inhibitor of the nuclear transport protein XPO1 enhances the anticancer efficacy of KRAS G12C inhibitors in preclinical models of KRAS G12C mutant cancers 91%
- Activation of the mevalonate pathway in response to anti-cancer treatments drives glioblastoma recurrences through activation of Rac-1 90%
- A spatially resolved mechanistic growth law for cancer drug development. 89%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.