Fecal sample biobanking for breast cancer research focused on the gut microbiome

BackgroundGut microbiome is an emerging potentially modifiable contributor to breast health, including breast cancer (BCa). To advance prevention research in this area, we established a prospective biobanking cohort of cancer-free women. MethodsEligible women were [≥]40 years old, had no cancer history and no recent antibiotic use. Women were enrolled during screening mammography visits at three imaging centers in Florida (February 2021-June 2024), completed a BCa risk factor survey, and underwent body measurements. We collected digital mammograms and stool/urine/saliva samples. Optionally, women completed NIHs Diet History Questionnaire and a neighborhood stress questionnaire. Mammographic breast density (MBD) was assessed using established computerized approaches. ResultsWe recruited 733 cancer-free women (49% Caucasian, 21% African American, 26% Hispanic, and 4% from mixed/other races). The average age was 60 years (range 40-92); the majority (68.3%) were postmenopausal. BCa risk factor, neighborhood stress and diet questionnaires were completed by 97%, 65% and 58% of participants, respectively. Urine, saliva, and mammograms were available for all women; 83% also returned stool samples. ConclusionsWe have established a representative cohort of screen-aged women with comprehensive BCa risk factor data, biospecimen collection, and MBD. ImpactThis unique resource provides opportunity for future gut microbiome-focused BCa prevention research.