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Mammalian Cell Culture and Transfection in a Low-Cost, CO2-Independent Environment

Lamb, A.; Gagliardi, A.; Lorge, D.; Carillo, L. S.

2025-10-16 cell biology
10.1101/2025.10.14.682406 bioRxiv
Show abstract

Traditional mammalian cell culture requires CO2 incubation to maintain physiological pH, creating infrastructure costs that limit accessibility. We demonstrate successful HEK293T/17 culture using HEPES-buffered CO2-independent medium in a repurposed consumer-grade egg incubator, reducing incubator equipment costs by >98% while maintaining viability through 10+ passages. To assess feasibility for recombinant protein production, we performed plasmid transfection with pcDNA3.1(+)-EGFP via electroporation. Fluorescence microscopy confirmed robust EGFP expression within 48 hours. These results demonstrate that mammalian cell culture and genetic engineering for recombinant protein production can be achieved using cost-effective, widely available equipment rather than conventional high-cost laboratory infrastructure.

Matching journals

The top 10 journals account for 50% of the predicted probability mass.

1
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50% of probability mass above
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1.5%
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Top 0.5%
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26
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Top 0.5%
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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.