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TMEM147 promotes tumor progression by promoting immunosuppression and serves as a prognostic biomarker for lung adenocarcinoma

Ding, L.; Ding, Y.

2025-08-02 oncology
10.1101/2025.08.01.25332641 medRxiv
Show abstract

TMEM147, an ER membrane protein, is linked to lung adenocarcinoma (LUAD) but its role remains unclear. This study combines bioinformatics and experiments to explore TMEM147s function in LUAD progression. TMEM147 expression was analyzed using TCGA/GEO data and validated in LUAD cells. Survival analysis assessed its prognostic value. GO/KEGG and ssGSEA revealed functional pathways and immune microenvironment interactions. Transcription factor binding predictions and in vitro assays (migration, invasion, proliferation) evaluated TMEM147s role. TMEM147 was upregulated in LUAD and correlated with poor outcomes. FLI1 was predicted as a transcriptional regulator. TMEM147 influenced immune cell infiltration and was associated with ribonucleoprotein biogenesis and oxidative phosphorylation (OXPHOS) . Silencing TMEM147 reduced cancer cell migration, invasion, and proliferation, suggesting its potential as a biomarker and therapeutic target.

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