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Protocol for GDT, Gene Dictionary Tool, to create and implement a gene dictionary across annotated genomes

Dupin, B.; Lima, M. S.; Paschoal, A. R.; Smith, D. R.

2025-06-16 bioinformatics
10.1101/2025.06.15.659783 bioRxiv
Show abstract

The Gene Dictionary Tool is a protocol for the creation and implementation of gene dictionaries across annotated genomes. Through a Python CLI (Command Line Interface), the user identifies what annotations are missing in the inputted dictionary. Via two Jupyter Notebooks, the user builds a gene dictionary based on attributes retrieved from inputted GFF3 files. The final output, a .gdict file, is Findable, Accessible, Interoperable, and Reusable (FAIR). We showcase GDTs functionalities by creating a gene dictionary for fungal mitochondrial genomes.

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