An efficient protocol to derive primordial germ cell like cells in vitro from murine embryonic stem cells.
Kumar, U.; Kumar, N.; Shekar, P. C.; Thangaraj, K.
Show abstract
Primordial germ cells (PGCs) are the precursors of germ cells, specified from a subset of epiblast stem cells at the post-implantation stage of mammalian embryonic development. Over the past decade, primordial germ cell-like cells (PGCLCs) have been successfully derived in vitro from mouse and human embryonic stem cells (ESCs). These PGCLCs can further differentiate into mature sperm upon transplantation into neonatal testes. Such advancements offer promising strategies to address male infertility, which affects approximately 15% of couples worldwide. The discovery of induced pluripotent stem cells (iPSCs), which possess similar potential to ESCs, further raises hope for patient-specific therapeutic interventions. However, a major challenge remains: the low efficiency of in vitro PGCLC differentiation. To overcome this, we developed an optimized PGCLC differentiation strategy using a Dppa3-mCherry knock-in reporter mouse ESC line, TDM11. By testing various culture conditions, we identified ascorbic acid as a potent inducer of PGCLC differentiation from ESCs, providing a potential avenue for enhancing in vitro germ cell derivation.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Deciphering the heterogeneity of differentiating hPSC-derived corneal limbal stem cells through single-cell RNA-sequencing 96%
- Apoptosis, G1 phase stall and premature differentiation account for low chimeric competence of Human and rhesus monkey naive pluripotent stem cells 95%
- Mitochondrial state determines functionally divergent stem cell population in planaria 95%
Similar papers in this journal
- Unlocking cellular plasticity: Enhancing human iPSC reprogramming through bromodomain inhibition and extracellular matrix gene expression regulation 95%
- Arp2/3 mediated dynamic lamellipodia of the hPSC colony edges promote liposome-based DNA delivery 94%
- miR-183/96/182 cluster is an important morphogenetic factor targeting PAX6 expression in differentiating human retinal organoids 94%
Similar papers in this journal
Similar papers in this journal
- Endogenous suppression of WNT signalling in human embryonic stem cells leads to low differentiation propensity towards definitive endoderm 96%
- Generating an organ-deficient animal model using a multi-targeted CRISPR-Cas9 system 94%
- Dual SMAD inhibition and Wnt inhibition enhances the differentiation of induced pluripotent stem cells into Retinal Ganglion cells (iPSC-RGCs) 94%
Similar papers in this journal
- Generation of human induced pluripotent stem cell (hiPSC) lines from patients with extreme high and low polygenic scores for QT interval 95%
- Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936 94%
- Decoding molecular markers and transcriptional circuitry of naive and primed states of human pluripotency 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.