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Multiplex qPCR Assay for HIV-1 Proviral DNA Detection and Subtype Characterization: Exploiting Quenching of Multiple Fluorophores with a Single Quencher Operating in Trans
2025-03-14
hiv aids
Title + abstract only
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Accurate detection and quantification of HIV-1 proviral DNA are critical for effective patient monitoring and therapeutic decision-making. In this study, we developed a multiplexed quantitative PCR (qPCR) assay designed to detect HIV-1 proviral DNA, determine viral subtype, specifically identifying the predominant subtype C and validate assay performance using an internal control. Gene-specific primers were engineered by appending an 8-base biotag followed by a common 18-base sequence at the 5' ...
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