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Immunological and Antigenic Signatures Associated with Chronic Illnesses after COVID-19 Vaccination

Bhattacharjee, B.; Lu, P.; Monteiro, V. S.; Tabachnikova, A.; Wang, K.; Hooper, W. B.; Bastos, V.; Greene, K.; Sawano, M.; Guirgis, C.; Tzeng, T. J.; Warner, F.; Baevova, P.; Kamath, K.; Reifert, J.; Hertz, D.; Dressen, B.; Tabacof, L.; Wood, J.; Cooke, L.; Doerstling, M.; Nolasco, S.; Ahmed, A.; Proal, A.; Putrino, D.; Guan, L.; Krumholz, H.; Iwasaki, A.

2025-02-18 allergy and immunology
10.1101/2025.02.18.25322379 medRxiv
Show abstract

COVID-19 vaccines have prevented millions of COVID-19 deaths. Yet, a small fraction of the population reports a chronic debilitating condition after COVID-19 vaccination, often referred to as post- vaccination syndrome (PVS). To explore potential pathobiological features associated with PVS, we conducted a decentralized, cross-sectional study involving 42 PVS participants and 22 healthy controls enrolled in the Yale LISTEN study. Compared with controls, PVS participants exhibited differences in immune profiles, including reduced circulating memory and effector CD4 T cells (type 1 and type 2) and an increase in TNF+ CD8 T cells. PVS participants also had lower anti-spike antibody titers, primarily due to fewer vaccine doses. Serological evidence of recent Epstein-Barr virus (EBV) reactivation was observed more frequently in PVS participants. Further, individuals with PVS exhibited elevated levels of circulating spike protein compared to healthy controls. These findings reveal potential immune differences in individuals with PVS that merit further investigation to better understand this condition and inform future research into diagnostic and therapeutic approaches.

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