Species-specific detection of Schistosoma japonicum using the SNAILS DNA-based biosensor
Webb, A. J.; Zhao, Q.-P.; Allan, F.; Kelwick, R. J. R.; Emery, A. M.; Freemont, P. S.
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The neglected tropical disease schistosomiasis continues to be a global health concern, especially in low- and middle-income countries, with at least 250 million people infected worldwide and a further 779 million at risk of infection. Schistosoma japonicum, which is found in parts of South Asia, causes intestinal schistosomiasis in humans, as well as infecting up to forty other mammalian species. Therefore, novel diagnostics that can detect S. japonicum are desirable. In this study, we have further developed and refined the SNAILS DNA-based biosensor technology for the detection of schistosomes and have applied this to the specific detection of S. japonicum. Several new SNAILS probe pairs were developed, and our optimised design successfully detected and differentiated between genomic DNA isolated from laboratory-derived Schistosoma mansoni cercariae and S. japonicum cercariae isolated from sites in the Peoples Republic of China.
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