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Sustainable and portable CRISPR-based diagnostics for high-sensitivity Mpox detection

Hirano, R.; Yoshimi, K.; Asano, K.; Takeshita, K.; Ishii, K. J.; Sato, K.; Mashimo, T.

2024-11-23 infectious diseases
10.1101/2024.11.20.24317678 medRxiv
Show abstract

Mpox has emerged as a critical public health challenge, creating an urgent need for rapid, reliable, and field-deployable diagnostic tools for outbreak settings. Here, we present Kairo-CONAN, a novel CRISPR-Cas3-based point-of-care (POC) diagnostic platform for Mpox, engineered for sustainability and portability. This system leverages a disposable hand warmer (Kairo) as a stable heat source and incorporates freeze-dried reagents for ambient temperature stability, enabling device-free, sensitive detection through lateral flow assay strips. Utilizing CRISPR-Cas3s unique DNA-targeting and cleavage properties, we optimized probe DNA configurations for high specificity and designed clade-specific target crRNAs. Kairo-CONAN demonstrated rapid, high-sensitivity, and specific detection of Mpox virus (MPXV) DNA across multiple clades, including Clade Ia (Congo), Clade Ib (synthetic DNA), and Clade IIb (Tokyo). By addressing logistical and environmental challenges, Kairo-CONAN offers a sustainable, cost-effective, and field-adapted solution for infectious disease diagnostics, aligning with the 100-day mission framework to enhance global outbreak response efforts.

Published in npj Biosensing · not in our set (fewer than 10 published preprints to learn from) · training set

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