Influence of formaldehyde on signaling pathways when used in mammalian cell culture
Ostmann, K.; Kraegeloh, A.; Weber, W.
Show abstract
Formaldehyde is the smallest existing aldehyde, a highly reactive color less gas at room temperature and ubiquitously present in our atmosphere. Because of its reactivity leading to the crosslinking of macromolecules like proteins, it is widely used in industrial applications, but also in cell biology in order to preserve cells and tissues for further analysis. In this work, we show that formaldehyde releasing solutions commonly used for fixation of cells, can diffuse via the gas phase to the neighboring well and influence signaling processes in the therein cultured cells. To analyze this effect, we utilized a stable reporter cell line for YAP signaling or a gene expression-based reporter for activation of the NF-{kappa}B pathway. Especially the stable reporter cell line can also be used as sensor for bioavailable formaldehyde. The observed impact of formaldehyde on cellular signaling underscores the need for careful planning of experimental protocols and emphasizes the importance of implementing proper controls when utilizing this reagent in cellular signaling analyses.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Functional and structural consequences of epithelial cell invasion by Bordetella pertussis adenylate cyclase toxin 94%
- Mitochondrial DNA is a sensitive surrogate and oxidative stress target in oral cancer cells 94%
- Effect of high-frequency low-intensity pulsed electric field on protecting SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury via ERK pathway 93%
Similar papers in this journal
- Arsenic hexoxide has differential effects on cell proliferation and genome-wide gene expression in human primary mammary epithelial and MCF7 cells 93%
- Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure 93%
- High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents 93%
Similar papers in this journal
- Synthetic and plant-derived multivalent galactans as modulators of cancer-associated galectins-3 and -9 92%
- The impact of alpha-synuclein aggregates on blood-brain barrier integrity in the presence of neurovascular unit cells 92%
- Antifungal therapeutic potential of Candida albicans Fun30: screening and validation of novel inhibitors against Candida albicans 91%
Similar papers in this journal
- Two-color live-cell STED nanoscopy by click labeling with cell-permeable fluorophores 92%
- Anti-apoptotic and anti-inflammatory properties of grapefruit IntegroPectin on human microglial HMC3 cell line 92%
- Ultrasound-mediated drug diffusion, uptake, and cytotoxicity in glioblastoma 3D tumour sphere model 92%
Similar papers in this journal
- Partial Activation of PPAR-gamma by Synthesized Quercetin Derivatives Modulates TGF-beta-Induced EMT in Lung Cancer cells 93%
- Systematic Transmission Electron Microscopy-Based Identification and 3D Reconstruction of Cellular Degradation Machinery 90%
- A Hierarchical Cascade of Organellar Silencing and their Regeneration under Anaesthetic Stress in Plants 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.