AD plasma biomarkers are stable for an extended period at negative 20 degrees: implications for resource-constrained environments.
Ayele, B. A.; Whitehead, P. G.; Pascual, J.; Gu, T.; Arvizu, J.; Golightly, C. G.; Adams, L. D.; Pericak-Vance, M. A.; Vance, J. M.; Griswold, A. J.
Show abstract
Standard procedures for measuring Alzheimers disease (AD) plasma biomarkers include storage at -80{degrees}C. This is challenging in countries lacking research infrastructure, such -80{degrees}C freezer. To investigate stability of AD biomarkers from plasma stored at -20{degrees}C, we compared aliquots stored at -80{degrees}C and others at -20{degrees}C for two, four, six, fifteen, and thirty-five weeks. pTau181, A{beta}42, A{beta}40, NfL, and GFAP were measured for each timepoint. pTau181 and A{beta}42/A{beta}40 ratios showed minimal variation for up to 15 weeks. NfL and GFAP had higher variability. This finding of 15-week stability at -20{degrees}C enables greater participation in AD biomarker studies in resource constrained environments.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- The Association of Alzheimer’s Disease-related Blood-based Biomarkers with Cognitive Screening Test Performance in the Congolese Population in Kinshasa 96%
- Differences between plasma and CSF p-tau181 and p-tau231 in early Alzheimer’s disease 96%
- CSF sphingomyelins in Alzheimer’s disease, neurodegeneration, and neuroinflammation 94%
Similar papers in this journal
- Association of Item-Level Responses to Cognitive Function Index with Tau Pathology and Hippocampal volume in The A4 Study 95%
- Plasma p-tau181/Aβ 1-42 ratio predicts Aβ-PET status and correlates with CSF-p-tau181/Aβ 1-42 and future cognitive decline 94%
- CSF metabolites associate with CSF tau and improve prediction of Alzheimer's disease status 94%
Similar papers in this journal
- An immuno-enrichment free, validated quantification of tau protein in human CSF by LC-MS/MS 94%
- Leveraging large multi-center cohorts of Alzheimer Disease endophenotypes to understand the role of Klotho heterozygosity on disease risk 94%
- NMR Analysis of the Correlation of Metabolic Changes in Blood and Cerebrospinal Fluid in Alzheimer Model Male and Female Mice 93%
Similar papers in this journal
- Targeted Serum Metabolomic Profiling and Machine Learning Approach in Alzheimer’s Disease using the Alzheimer’s Disease Diagnostics Clinical Study (ADDIA) Cohort 94%
- Blood Biomarkers for Diagnosis & Differential Diagnosis of Alzheimers Disease in Real-World Clinical Populations: A Systematic Review 94%
- Association of GLOD4 with Alzheimers Disease in Humans and Mice 93%