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Chromosomal-level genome assembly of the long-spined sea urchin Diadema setosum (Leske, 1778)

Hong Kong Biodiversity Genomics Consortium, ; Hui, J. H. L.; Chan, T.-F.; Chan, L.; Cheung, S. G.; Cheang, C. C.; Fang, J.; Gaitan-Espitia, J. D.; Lau, S.; Sung, Y. H.; Wong, C.; Yip, K.; Wei, Y.; So, W. L.; Nong, W.; Chui, A. P. Y.; Fong, T. H. W.; Yip, H. Y.

2024-01-18 genomics
10.1101/2024.01.16.575490 bioRxiv
Show abstract

The long-spined sea urchin Diadema setosum is an algal and coral feeder widely distributed in the Indo-Pacific and can cause severe bioerosion on the reef community. Nevertheless, the lack of genomic information has hindered the study its ecology and evolution. Here, we report the chromosomal-level genome (885.8 Mb) of the long-spined sea urchin D. setosum using a combination of PacBio long-read sequencing and Omni-C scaffolding technology. The assembled genome contained scaffold N50 length of 38.3 Mb, 98.1 % of BUSCO (Geno, metazoa_odb10) genes, and with 98.6% of the sequences anchored to 22 pseudo-molecules/chromosomes. A total of 27,478 genes including 23,030 protein-coding genes were annotated. The high-quality genome of D. setosum presented here provides a significant resource for further understanding on the ecological and evolutionary studies of this coral reef associated sea urchin.

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