Beyond Antibiotics: Cinnamic Acid's role in combatting complex biofilms in the oral cavity

Traditional antibiotic therapy has become inefficient in treating oral plaques, due to the the presence of highly adherent bacteria in complex infections of oral cavities. Our study aimed to evaluate the anti-biofilm activity of Cinnamic Acid against single and multispecies bacteria causing dental plaques. Different concentrations of cinnamic acid (1-1000 mg/L) were tested using Biofilm assays, EPS analysis, and biomass quantification. To gain insight, a drug-likeness chemoinformatics study of cinnamic acid was conducted. We found that lower concentrations (1-400mg/L) of Cinnamic Acid had a minor effect on biofilm formation inhibition. Whilst concentrations of 600mg/L and above had shown significant biofilm production, EPS production and biomass production reduced down to 80%.Cinnamic Acid has proven to contain antibacterial, antibiofilm, and drug-likeness properties and is a prominent compound in combating oral biofilm. Authors SummaryCombating oral pathogenic biofilm is an important approach in treating polymicrobial oral infections, We have tested the anti-biofilm activity of cinnamic acid at different connections (1-1000 mg/L) and against single and multispecies biofilm forming bacteria. We found that cinnamic acid had a notable reduction in biofilm formation and EPS production, and reduced total biofilm biomass. Cinnamic Also fulfills the lipinski ROFs criteria for drug-likeness. Due to its antibacterial, anti-biofilm, and drug-likeness properties, a plant-derived compound such as Cinnamic Acid can be a new prominent approach to defecting the global risk of oral pathogenic biofilms. And serve as an alternative to conventional oral care methods which have been rendered inefficient by biofilm-forming pathogens.