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Substantial decline of phasic dopamine signaling in senescent male rats does not impact dopamine-dependent Pavlovian conditioning.

Sandberg, S. G.; Stanford, C. A.; Phillips, P. E. M.

2023-12-23 animal behavior and cognition
10.1101/2023.12.21.572806 bioRxiv
Show abstract

Normal aging is associated with cognitive decline which impacts financial decision making. One of the underlying features of decision making is probability estimation, in which nucleus accumbens dopamine signaling has been implicated. Here we used fast-scan cyclic voltammetry to probe for age differences in dopamine signaling, and pharmacological manipulation to test for age differences in the dopamine dependence of Pavlovian conditioning. We found differences in phasic dopamine signaling to reward delivery, and unconditioned and conditioned stimuli, but no difference in conditioned approach between adult and senescent groups. In addition, we found that dopamine receptor antagonism with flupenthixol (225 g/kg, i.p.) partially inhibited conditioned approach in the adult group, whereas it completely blocked conditioned approach in the senescent group. Further increase in concentration to 300 g/kg, i.p. resulted in complete inhibition of conditioned approach behavior in both age groups. Therefore, while phasic dopamine signaling in the nucleus accumbens of senescent animals is greatly diminished in concentration, these animals maintain dopamine dependent Pavlovian conditioning.

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